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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >L-arginine uptake in rat cerebral mitochondria.
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L-arginine uptake in rat cerebral mitochondria.

机译：大鼠脑线粒体中L-精氨酸的摄取。

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摘要

The kinetics of L-[14C]arginine (L-[14C]Arg) uptake and the effects of potential competitors on the uptake were analysed in nonsynaptic mitochondria isolated from rat cerebral hemispheres. Analysis of uptake kinetics revealed a high affinity component with a mean Km = 0.08 mM, and Vmax = 1.89 nmoles/min/mg, and a very low affinity component probably manifesting diffusion. The uptake of 25 mM L-Arg was strongly inhibited by a 20-fold excess of L-lysine (L-Lys) and L-ornithine (L-Orn), but not by D-Arg nor any neutral amino acid, which resembles the characteristics of the gamma+ transport system operating in the nerve- and glia cell-, and synaptic plasma membranes. Also in consistance with the other gamma+ systems, L-Arg uptake to mitochondria was inhibited by a nitric oxide synthase (NOS) inhibitor L-N-monomethyl arginine (L-NMMA), but not by another NOS inhibitor NG-nitro-L-arginine (L-NNA). However, the uptake was very little affected by 20-fold excess of L-histidine (L-His), L-glutamate (L-Glu) or L-glutamine (L-Gln), which is in contrast to the nonmitochondrial systems. The uptake was only marginally influenced by cytoplasmic L-Arg metabolites: ammonia, creatine, putrescine, or the mitochondrial L-Arg decarboxylation product, agmatine.
机译：在大鼠脑半球分离的非突触线粒体中，分析了L- [14C]精氨酸（L- [14C] Arg）摄取的动力学以及潜在竞争者对摄取的影响。摄取动力学分析表明，高亲和力组分的平均Km = 0.08 mM，Vmax = 1.89 nmoles / min / mg，而非常低的亲和力组分则可能表现出扩散。 L-赖氨酸（L-Lys）和L-鸟氨酸（L-Orn）过量20倍，强烈抑制了25 mM L-Arg的摄取，但D-Arg或任何类似的中性氨基酸均没有抑制在神经和神经胶质细胞以及突触质膜中运作的gamma +转运系统的特征。同样与其他gamma +系统一致，一氧化氮合酶（NOS）抑制剂LN-单甲基精氨酸（L-NMMA）抑制了L-Arg对线粒体的吸收，但另一种NOS抑制剂NG-硝基-L-精氨酸（ L-NNA）。但是，与非线粒体系统相反，摄取的L-组氨酸（L-His），L-谷氨酸（L-Glu）或L-谷氨酰胺（L-Gln）几乎没有受到20倍的影响。摄取仅受到细胞质L-Arg代谢产物的轻微影响：氨，肌酸，腐胺或线粒体L-Arg脱羧产物胍丁胺。

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