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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Ceruloplasmin expression and its role in iron transport in C6 cells.
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Ceruloplasmin expression and its role in iron transport in C6 cells.

机译：铜蓝蛋白表达及其在C6细胞中铁转运中的作用。

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摘要

Ceruloplasmin (CP) is essential for brain iron homeostasis. However, little is known about the effect of iron on CP expression in the brain. Also, the role of CP in brain iron transport has not been well determined. In this study, we investigated the effects of iron on CP expression and the role of CP in iron transport in the C6 rat glioma cells. Our data showed that treatment of the cells with iron (cell iron overload) or iron chelators (cell iron deficiency) did not induce a significant change in the expression of CP mRNA. However, western blotting analysis demonstrated that cell iron overload induced a significant decrease in CP protein content in the cells and that treatment with iron chelators led to a significant increase in CP protein level in the cells. These findings suggest a translational regulation of CP expression by iron in the cells. We also examined the effects of CP on iron transport in the cells. We found that glycosylphosphatidylinositol-anchored CP did not have any impact on iron uptake by normal iron or iron-deficient cells nor on iron release from normal iron or iron-sufficient cells. However, low concentrations of soluble CP (2-8 microg/ml) increased iron uptake by iron-deficient C6 glioma cells, while the same concentrations of CP had no effect on iron uptake by normal iron cells and iron release from normal iron and iron-sufficient cells. The possible reason for the difference between our results in vitro and those obtained from in vivo studies was discussed.
机译：铜蓝蛋白（CP）对于脑铁稳态至关重要。但是，关于铁对脑中CP表达的影响知之甚少。而且，CP在脑铁运输中的作用还没有很好地确定。在这项研究中，我们调查了铁对CP表达的影响以及CP在C6大鼠神经胶质瘤细胞中铁转运中的作用。我们的数据显示，用铁（细胞铁超负荷）或铁螯合剂（细胞铁缺乏症）处理细胞不会引起CP mRNA表达的显着变化。但是，蛋白质印迹分析表明，细胞铁超负荷导致细胞中CP蛋白含量显着降低，铁螯合剂处理导致细胞中CP蛋白水平显着提高。这些发现表明细胞中铁对CP表达的翻译调控。我们还检查了CP对细胞中铁转运的影响。我们发现，糖基磷脂酰肌醇固定的CP对正常铁或缺铁细胞的铁摄取没有任何影响，也对正常铁或铁充足的细胞释放铁没有任何影响。但是，低浓度的可溶性CP（2-8 microg / ml）会增加缺铁的C6胶质瘤细胞对铁的吸收，而相同浓度的CP对正常铁细胞对铁的吸收以及正常铁和铁的铁释放没有影响。 -足够的细胞。讨论了我们的体外研究结果与体内研究结果之间差异的可能原因。

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