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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Type 2 metabotropic glutamate receptor (mGluR2) fails to negatively couple to cGMP in stably transfected cells.
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Type 2 metabotropic glutamate receptor (mGluR2) fails to negatively couple to cGMP in stably transfected cells.

机译：2型代谢型谷氨酸受体（mGluR2）在稳定转染的细胞中未能与cGMP负偶联。

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摘要

The group II metabotropic glutamate receptors 2 and 3 (mGluR2 and mGluR3) share sequence homology, common pharmacology and negative coupling to cAMP. We recently discovered that mGluR3 also is negatively coupled through a G-protein to the cGMP transduction pathway in rat cerebellar granule cells and astrocytes. To test the hypothesis that mGluR2 also has access to the cGMP pathway, C6 glioma cells were stably transfected with mGluR2 and mGluR3 cDNA and their coupling to cGMP levels was characterized. In contrast to many other cell lines, C6 has a robust cGMP response that makes it attractive in the study of receptor coupling to this second messenger pathway. Consistent with prior studies, the mGluR3 receptor was negatively coupled to cGMP and this coupling was blocked by PTX. In contrast, mGluR2 agonists failed to reduce sodium nitroprusside stimulated cGMP levels in transfected cell lines where the receptor was negatively coupled to cAMP. These data provide further support for the functional divergence between these two closely related receptors.
机译：II组代谢型谷氨酸受体2和3（mGluR2和mGluR3）具有序列同源性，共同的药理作用和与cAMP的负偶联。我们最近发现，mGluR3还通过G蛋白与大鼠小脑颗粒细胞和星形胶质细胞中的cGMP转导途径负相关。为了检验mGluR2也可以进入cGMP途径的假说，用mGluR2和mGluR3 cDNA稳定转染了C6胶质瘤细胞，并表征了它们与cGMP水平的偶联。与许多其他细胞系相比，C6具有强大的cGMP反应，使其在研究与第二种信使途径偶联的受体方面具有吸引力。与先前的研究一致，mGluR3受体与cGMP负偶联，并且该偶联被PTX阻断。相比之下，mGluR2激动剂在受体与cAMP负偶联的转染细胞系中未能降低硝普钠刺激的cGMP水平。这些数据为这两个密切相关的受体之间的功能差异提供了进一步的支持。

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