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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Acidosis and astrocyte amino acid metabolism.
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Acidosis and astrocyte amino acid metabolism.

机译：酸中毒和星形胶质细胞氨基酸代谢。

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摘要

The relationship between acidosis and the metabolism of glutamine and glutamate was studied in cultured astrocytes. Acidification of the incubation medium was associated with an increased formation of aspartate from glutamate and glutamine. The rise of the intracellular content of aspartate was accompanied by a significant decline in the extracellular concentration of both lactate and citrate. Studies with either [2-(15)N]glutamine or [15N]glutamate indicated that there occurred in acidosis an increased transamination of glutamate to aspartate. Studies with L-[2,3,3,4,4-(2)H5]glutamine indicated that in acidosis glutamate carbon was more rapidly converted to aspartate via the tricarboxylic acid cycle. Acidosis appears to result in increased availability of oxaloacetate to the aspartate aminotransferase reaction and, consequently, increased transamination of glutamate. The expansion of the available pool of oxaloacetate probably reflects a combination of: (a) Restricted flux through glycolysis and less production from pyruvate of acetyl-CoA, which condenses with oxaloacetate in the citrate synthetase reaction; and (b) Increased oxidation of glutamate and glutamine through a portion of the tricarboxylic acid cycle and enhanced production of oxaloacetate from glutamate and glutamine carbon. The data point to the interplay of the metabolism of glucose and that of glutamate in these cells.
机译：在培养的星形胶质细胞中研究了酸中毒与谷氨酰胺和谷氨酸代谢之间的关系。孵育培养基的酸化与谷氨酸和谷氨酰胺中天冬氨酸形成的增加有关。天冬氨酸细胞内含量的增加伴随着乳酸和柠檬酸的细胞外浓度的显着下降。用[2-（15）N]谷氨酰胺或[15N]谷氨酸的研究表明，在酸中毒中谷氨酸转为天冬氨酸的转氨作用增加。用L- [2,3,3,4,4-（2）H5]谷氨酰胺进行的研究表明，在酸中毒中，谷氨酸通过三羧酸循环更迅速地转化为天冬氨酸。酸中毒似乎导致草酰乙酸酯对天冬氨酸转氨酶反应的利用率增加，因此谷氨酸的转氨作用增加。草酰乙酸的可用库的扩大可能反映了以下因素的组合：（a）通过糖酵解的通量受到限制，以及丙酮酸乙二酸酯在柠檬酸合成酶反应中冷凝的乙酰辅酶A的丙酮酸产生的产量减少; （b）通过一部分三羧酸循环增加了谷氨酸和谷氨酰胺的氧化，并提高了由谷氨酸和谷氨酰胺碳生产草酰乙酸的能力。数据指向这些细胞中葡萄糖代谢和谷氨酸代谢的相互作用。

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