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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Effect of ethanol on ATP-induced phospholipases C and D and serine base exchange in glioma C6 cells.
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Effect of ethanol on ATP-induced phospholipases C and D and serine base exchange in glioma C6 cells.

机译：乙醇对神经胶质瘤C6细胞中ATP诱导的磷脂酶C和D以及丝氨酸碱基交换的影响。

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The effect of extracellular ATP, a nucleotide receptor agonist in the central nervous system, was investigated in glioma C6 cells on the intracellular Ca2+ level and the formation of phosphatidylethanol and phosphatidic acid in the presence and absence of ethanol (150 mM). In the cells prelabeled with [14C]palmitic acid, 100 microM ATP induced both the hydrolysis and the transphosphatidylation reactions leading to the formation of [14C]phosphatidic acid; addition of ethanol generated [14C]phosphatidylethanol. However, ATP-mediated increase in the level of [14C]phosphatidic acid was not inhibited by ethanol. Furthermore, ethanol augmented ATP-induced transient and sustained increase in the intracellular Ca2+ concentration, whereas ethanol alone did not produce any change in the intracellular Ca2+ level. These results indicate that in glioma C6 cells, ATP induces activation of polyphosphoinositide-specific phospholipase C and phospholipase D and that ethanol enhances this effect. In the present investigation we have also shown that long-term (2 days) ethanol treatment, at concentration relevant to chronic alcoholism (100 mM), decreased the incorporation of [14C]serine into phosphatidylserine. Since the effect of ethanol on ATP-induced activities of phospholipase C and phospholipase D and on serine base-exchange in glioma C6 cells differs significantly from that in cultured neuronal cells, these results may contribute to a better understanding of the mechanisms of ethanol action in cells of glial origin.
机译：在存在和不存在乙醇（150 mM）的情况下，在神经胶质瘤C6细胞中研究了细胞外ATP（中枢神经系统中的核苷酸受体激动剂）对细胞内Ca2 +水平以及磷脂酰乙醇和磷脂酸形成的影响。在预先标记有[14C]棕榈酸的细胞中，100 microM ATP诱导了水解反应和转磷​​脂酰化反应，导致[14C]磷脂酸的形成。加入乙醇生成[14C]磷脂酰乙醇。但是，乙醇不会抑制ATP介导的[14C]磷脂酸水平的增加。此外，乙醇会增加ATP诱导的细胞内Ca2 +浓度的瞬时和持续增加，而仅乙醇不会产生细胞内Ca2 +水平的任何变化。这些结果表明，在神经胶质瘤C6细胞中，ATP诱导了多磷酸肌醇特异性磷脂酶C和磷脂酶D的活化，并且乙醇增强了这种作用。在本研究中，我们还表明，与慢性酒精中毒（100 mM）有关的长期（2天）乙醇治疗可降低[14C]丝氨酸向磷脂酰丝氨酸的掺入。由于乙醇对神经胶质瘤C6细胞中ATP诱导的磷脂酶C和磷脂酶D的活性以及丝氨酸碱基交换的影响与培养的神经元细胞有显着差异，因此这些结果可能有助于更好地了解乙醇对神经胶质细胞的作用机理。胶质细胞起源。

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