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STRUCTURE OF THE THIRD CYTOPLASMIC LOOP OF BOVINE RHODOPSIN

机译：牛视紫红质第三胞质环的结构

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The three-dimensional high-resolution structure of rhodopsin is unknown, as is the case for almost all integral membrane proteins. As part of an alternative approach to determination of membrane protein structure, we are pursuing the structure of cytoplasmic domains of this G-protein receptor. A peptide, rhoIII, with the sequence of the third cytoplasmic loop of bovine rhodopsin was synthesized. This soluble peptide was biologically active, inhibiting the Light-stimulated activation of the rod cell phosphodiesterase by rhodopsin in rod outer segment disks. Therefore rhoIII likely contains structural elements characteristic of native rhodopsin. The solution structure of rhoIII was determined by H-1 nuclear magnetic resonance. A defined structure was obtained for about 70% of rhoIII. A model of a turn-helix-turn motif could then be proposed for the third cytoplasmic loop of rhodopsin, which suggested a molecular switch for activation of the G-protein by the receptor. [References: 19]
机译：视紫红质的三维高分辨率结构是未知的，几乎所有完整的膜蛋白都是如此。作为确定膜蛋白结构的另一种方法，我们正在寻求该G蛋白受体胞质结构域的结构。合成了肽rhoIII，其具有牛视紫红质的第三个胞质环的序列。该可溶性肽具有生物活性，可抑制视紫红质在视杆外节段盘中刺激视杆细胞磷酸二酯酶的激活。因此，rhoIII可能含有天然视紫红质的结构元素。通过H-1核磁共振确定rhoIII的溶液结构。对于约70％的rhoIII获得确定的结构。然后可以为视紫红质的第三个胞质环提出一个螺旋-螺旋-转体基序的模型，这提示了通过受体激活G蛋白的分子开关。 [参考：19]

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