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首页> 外文期刊>Neuropathology and applied neurobiology >GDNF-treated acellular nerve graft promotes motoneuron axon regeneration after implantation into cervical root avulsed spinal cord
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GDNF-treated acellular nerve graft promotes motoneuron axon regeneration after implantation into cervical root avulsed spinal cord

机译：GDNF处理的脱细胞神经移植物植入颈根撕脱脊髓后可促进运动神经元轴突再生

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摘要

It is well known that glial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor for motoneurons. We have previously shown that it greatly enhanced motoneuron survival and axon regeneration after implantation of peripheral nerve graft following spinal root avulsion. Aims: In the current study, we explore whether injection of GDNF promotes axon regeneration in decellularized nerve induced by repeated freeze-thaw cycles. Methods: We injected saline or GDNF into the decellularized nerve after root avulsion in adult Sprague-Dawley rats and assessed motoneuron axon regeneration and Schwann cell migration by retrograde labelling and immunohistochemistry. Results: We found that no axons were present in saline-treated acellular nerve whereas Schwann cells migrated into GDNF-treated acellular nerve grafts. We also found that Schwann cells migrated into the nerve grafts as early as 4 days after implantation, coinciding with the first appearance of regenerating axons in the grafts. Application of GDNF outside the graft did not induce Schwann cell infiltration nor axon regeneration into the graft. Application of pleiotrophin, a trophic factor which promotes axon regeneration but not Schwann cell migration, did not promote axon infiltration into acellular nerve graft. Conclusions: We conclude that GDNF induced Schwann cell migration and axon regeneration into the acellular nerve graft. Our findings can be of potential clinical value to develop acellular nerve grafting for use in spinal root avulsion injuries.
机译：众所周知，胶质细胞系衍生的神经营养因子（GDNF）是运动神经元的有效神经营养因子。先前我们已经表明，它在脊神经根撕脱术后植入周围神经移植物后大大增强了运动神经元的存活和轴突再生。目的：在当前的研究中，我们探讨了GDNF的注射是否能促进反复冻融循环诱导的脱细胞神经轴突再生。方法：我们在成年Sprague-Dawley大鼠根部撕脱伤后向脱细胞的神经注射了盐水或GDNF，并通过逆行标记和免疫组织化学评估了运动神经元轴突再生和Schwann细胞迁移。结果：我们发现在盐水处理的脱细胞神经中没有轴突，而施旺细胞迁移到了GDNF处理的脱细胞神经移植物中。我们还发现，雪旺细胞最早在植入后4天就迁​​移到了神经移植物中，这与移植物中再生轴突的首次出现相吻合。在移植物外使用GDNF不会诱导雪旺氏细胞浸润，也不会导致轴突再生到移植物中。促营养素（促进轴突再生但不促进雪旺氏细胞迁移的营养因子）的应用并未促进轴突浸润到脱细胞神经移植物中。结论：我们得出结论，GDNF诱导雪旺细胞迁移和轴突再生进入脱细胞神经移植物中。我们的发现可能具有潜在的临床价值，以开发用于脊柱根部撕脱伤的脱细胞神经移植。

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