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首页> 外文期刊>Biochemistry >OLIGONUCLEOTIDE-DIRECTED SWITCHING OF DNA POLYMERASES TO A DEAD-END TRACK
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OLIGONUCLEOTIDE-DIRECTED SWITCHING OF DNA POLYMERASES TO A DEAD-END TRACK

机译：寡核苷酸定向的DNA聚合酶切换至死轨道

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摘要

During DNA replication, the presence of oligonucleotides with partial homology to the template strand was shown to induce a switch of the polymerase from the normal template to the oligonucleotide. The latter acted as a dead-end template and led to abortive replication. The only prerequisite was that the oligonucleotide could form 7-9 base pairs with the newly synthesized DNA strand in order to switch templates. The switch occurred when base pairing of the oligonucleotide could take place with the 3'-end of the newly synthesized strand. These results show that oligonucleotides used in antisense or antigene strategies could have unexpected effects on replication. In addition, oligonucleotide-directed abortive replication might play an inhibitory role during PCR experiments on long DNA templates and lead to the amplification of truncated fragments. [References: 25]
机译：在DNA复制过程中，与模板链具有部分同源性的寡核苷酸的存在显示出可诱导聚合酶从正常模板向寡核苷酸的转换。后者充当了死胡同的模板，并导致了异常复制。唯一的先决条件是寡核苷酸可以与新合成的DNA链形成7-9个碱基对，以便切换模板。当寡核苷酸的碱基配对可以与新合成的链的3'末端发生时发生切换。这些结果表明，用于反义或抗原性策略的寡核苷酸可能会对复制产生意想不到的影响。此外，寡核苷酸指导的流产复制可能在长DNA模板的PCR实验中起抑制作用，并导致截短片段的扩增。 [参考：25]

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