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首页> 外文期刊>Neuron >Tweek, an evolutionarily conserved protein, is required for synaptic vesicle recycling.
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Tweek, an evolutionarily conserved protein, is required for synaptic vesicle recycling.

机译：突触小泡循环需要Tweek，一种进化上保守的蛋白质。

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摘要

Synaptic vesicle endocytosis is critical for maintaining synaptic communication during intense stimulation. Here we describe Tweek, a conserved protein that is required for synaptic vesicle recycling. tweek mutants show reduced FM1-43 uptake, cannot maintain release during intense stimulation, and harbor larger than normal synaptic vesicles, implicating it in vesicle recycling at the synapse. Interestingly, the levels of a fluorescent PI(4,5)P(2) reporter are reduced at tweek mutant synapses, and the probe is aberrantly localized during stimulation. In addition, various endocytic adaptors known to bind PI(4,5)P(2) are mislocalized and the defects in FM1-43 dye uptake and adaptor localization are partially suppressed by removing one copy of the phosphoinositide phosphatase synaptojanin, suggesting a role for Tweek in maintaining proper phosphoinositide levels at synapses. Our data implicate Tweek in regulating synaptic vesicle recycling via an action mediated at least in part by the regulation of PI(4,5)P(2) levels or availability at the synapse.
机译：突触小泡内吞作用对于在强烈刺激下维持突触通讯至关重要。在这里，我们描述了Tweek，突触小泡循环所需的保守蛋白。 tweek突变体显示FM1-43摄取减少，在强烈刺激下无法维持释放，并且比正常的突触小泡更大，其与突触小泡的循环有关。有趣的是，荧光PI（4,5）P（2）报告基因的水平在t周突变突触处降低，并且探针在刺激过程中异常定位。此外，已知与PI（4,5）P（2）结合的各种内吞衔接子是错误定位的，并且通过删除一个拷贝的磷酸肌醇磷酸酶突触木脂蛋白可以部分抑制FM1-43染料摄取和衔接子定位的缺陷，从而提示在突触中保持适当的磷酸肌醇水平。我们的数据暗示Tweek通过至少部分由PI（4,5）P（2）水平或突触可用性调节的作用来调节突触小泡循环。

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