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首页> 外文期刊>Neuron >Multicolor 'DiOlistic' labeling of the nervous system using lipophilic dye combinations.
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Multicolor 'DiOlistic' labeling of the nervous system using lipophilic dye combinations.

机译:使用亲脂性染料组合对神经系统进行多色“ DiOlistic”标记。

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摘要

We describe a technique for rapid labeling of a large number of cells in the nervous system with many different colors. By delivering lipophilic dye-coated particles to neuronal preparations with a "gene gun," individual neurons and glia whose membranes are contacted by the particles are quickly labeled. Using particles that are each coated with different combinations of various lipophilic dyes, many cells within a complex neuronal network can be simultaneously labeled with a wide variety of colors. This approach is most effective in living material but also labels previously fixed material. In living material, labeled neurons continue to show normal synaptic responses and undergo dendritic remodeling. This technique is thus useful for studying structural plasticity of neuronal circuits in living preparations. In addition, the Golgi-like labeling of neurons with many different colors provides a novel way to study neuronal connectivity.
机译:我们描述了一种以多种不同颜色快速标记神经系统中大量细胞的技术。通过用“基因枪”将亲脂性染料包被的颗粒递送至神经元制剂,其膜与颗粒接触的单个神经元和神经胶质细胞被快速标记。使用各自涂有各种亲脂性染料不同组合的颗粒,可以同时用多种颜色标记复杂神经元网络中的许多细胞。这种方法对于活体材料最有效,但也可以标记以前固定的材料。在生物材料中,标记的神经元继续显示正常的突触反应并经历树突状重塑。因此,该技术可用于研究活体制剂中神经元回路的结构可塑性。另外,具有许多不同颜色的神经元的高尔基样标记提供了一种研究神经元连通性的新颖方法。

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