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首页> 外文期刊>Neuron >Assembly of new individual excitatory synapses: time course and temporal order of synaptic molecule recruitment (see comments)
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Assembly of new individual excitatory synapses: time course and temporal order of synaptic molecule recruitment (see comments)

机译：新的个体兴奋性突触的组装：突触分子募集的时间过程和时间顺序（请参阅评论）

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摘要

Time-lapse microscopy, retrospective immunohistochemistry, and cultured hippocampal neurons were used to determine the time frame of individual glutamatergic synapse assembly and the temporal order in which specific molecules accumulate at new synaptic junctions. New presynaptic boutons capable of activity-evoked vesicle recycling were observed to form within 30 min of initial axodendritic contact. Clusters of the presynaptic active zone protein Bassoon were present in all new boutons. Conversely, clusters of the postsynaptic molecule SAP90/PSD-95 and glutamate receptors were found on average only approximately 45 min after such boutons were first detected. AMPA- and NMDA-type glutamate receptors displayed similar clustering kinetics. These findings suggest that glutamatergic synapse assembly can occur within 1-2 hr after initial contact and that presynaptic differentiation may precede postsynaptic differentiation.
机译：延时显微镜，回顾性免疫组织化学和培养的海马神经元用于确定单个谷氨酸能突触组装的时间框架和特定分子在新的突触连接处积累的时间顺序。观察到在初次轴突接触后30分钟内会形成新的突触前突触，这些突触能引起囊泡回收。突触前活性区蛋白Bassoon的簇存在于所有新的钮扣中。相反，在首次检测到这种纽扣后大约45分钟，平均发现突触后分子SAP90 / PSD-95和谷氨酸受体的簇。 AMPA型和NMDA型谷氨酸受体表现出相似的聚集动力学。这些发现表明，谷氨酸能突触组装可在最初接触后1-2小时内发生，并且突触前分化可先于突触后分化。

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