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首页> 外文期刊>Neuropathology: official journal of the Japanese Society of Neuropathology >Immunocytochemical analysis of glucose transporter protein-1 (GLUT-1) in typical, brain invasive, atypical and anaplastic meningioma
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Immunocytochemical analysis of glucose transporter protein-1 (GLUT-1) in typical, brain invasive, atypical and anaplastic meningioma

机译：典型，脑浸润性，非典型和间变性脑膜瘤中葡萄糖转运蛋白-1（GLUT-1）的免疫细胞化学分析

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摘要

Glucose transporter-1 (GLUT-1) is one of the major isoforms of the family of glucose transporter proteins that facilitates the import of glucose in human cells to fuel anaerobic metabolism. The present study was meant to determine the extent of the anaerobic/hypoxic state of the intratumoral microenvironment by staining for GLUT-1 in intracranial non-embolized typical (WHO grade I; n=40), brain invasive and atypical (each WHO grade II; n=38) and anaplastic meningiomas (WHO grade III, n=6). In addition, GLUT-1 staining levels were compared with the various histological criteria used for diagnosing WHO grade II and III meningiomas, namely, brain invasion, increased mitotic activity and atypical cytoarchitectural change, defined by the presence of at least three out of hypercellularity, sheet-like growth, prominent nucleoli, small cell change and spontaneous necrosis. The level of tumor hypoxia was assessed by converting the extent and intensity of the stainings by multiplication in an immunoreactive score (IRS) and statistically evaluated. The results were as follows. (1) While GLUT-1 expression was found to be mainly weak in WHO grade I meningiomas (IRS=1-4) and to be consistently strong in WHO grade III meningiomas (IRS=6-12), in WHO grade II meningiomas GLUT-1 expression was variable (IRS=1-9). (2) Histologically typical, but brain invasive meningiomas (WHO grade II) showed no or similarly low levels of GLUT-1 expression as observed in WHO grade I meningiomas (IRS=0-4). (3) GLUT-1 expression was observed in the form of a patchy, multifocal staining reaction in 76% of stained WHO grade I-III meningiomas, while diffuse staining (in 11%) and combined multifocal and areas of diffuse staining (in 13%) were only detected in WHO grades II and III meningiomas, except for uniform staining in angiomatous WHO grade I meningioma. (4) Spontaneous necrosis and small cell change typically occurred away from the intratumoral capillary network embedded within the pattern of GLUT-1 staining. Taken together, GLUT-1 staining cannot be applied as a substitute for histologic grading in order to predict tumor behavior. However, assessment of tumor hypoxia in association with spontaneous necrosis and foci of small cell change may substantially contribute to the neuropathologic diagnosis of WHO grades II and III meningioma.
机译：葡萄糖转运蛋白-1（GLUT-1）是葡萄糖转运蛋白家族的主要同工型之一，可促进人体细胞中葡萄糖的输入以促进厌氧代谢。本研究旨在通过对颅内非栓塞性典型（WHO I级; n = 40），脑浸润性和非典型性（每个WHO II级）的GLUT-1染色，确定肿瘤内微环境的无氧/低氧状态的程度; n = 38）和间变性脑膜瘤（WHO III级，n = 6）。此外，将GLUT-1染色水平与用于诊断WHO WHO II级和III级脑膜瘤的各种组织学标准（即脑浸润，有丝分裂活性增加和非典型细胞结构改变）进行了比较，这是由至少3种细胞过多性的存在所定义的，片状生长，突出的核仁，小细胞变化和自发性坏死。通过乘以免疫反应评分（IRS）转换染色的程度和强度来评估肿瘤缺氧的水平，并进行统计学评估。结果如下。 （1）虽然在WHO I级脑膜瘤（IRS = 1-4）中发现GLUT-1表达主要弱，而在WHO WHO III级脑膜瘤中（IRS = 6-12）GLUT-1表达始终很强，但在WHO WHO II级脑膜瘤中GLUT -1表达是可变的（IRS = 1-9）。 （2）从组织学上看，但脑浸润性脑膜瘤（WHO II级）与世界卫生组织I级脑膜瘤（IRS = 0-4）中观察到的GLUT-1表达水平无关或较低。 （3）在76％的WHO I-III级脑膜瘤中，GLUT-1的表达呈斑块状，多灶性染色反应，而弥漫性染色（11％）以及多灶和弥漫性染色区域（13种）相结合仅在WHO II级和III级脑膜瘤中检测到％），但在血管瘤性WHO I级脑膜瘤中均匀染色除外。 （4）自发性坏死和小细胞改变通常发生在GLUT-1染色模式内嵌入的瘤内毛细血管网络之外。综上所述，GLUT-1染色不能用作组织学分级的替代物，以预测肿瘤的行为。然而，评估肿瘤缺氧与自发性坏死和小细胞变化灶有关可能对WHO II和III级脑膜瘤的神经病理学诊断有重大贡献。

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