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首页> 外文期刊>Neuron >Habenula 'cholinergic' neurons corelease glutamate and acetylcholine and activate postsynaptic neurons via distinct transmission modes.
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Habenula 'cholinergic' neurons corelease glutamate and acetylcholine and activate postsynaptic neurons via distinct transmission modes.

机译：哈贝努拉的“胆碱能”神经元通过不同的传输模式共同释放谷氨酸和乙酰胆碱并激活突触后神经元。

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摘要

Acetylcholine is an important neurotransmitter, and the habenulo-interpeduncular projection is a major cholinergic pathway in the brain. To study the physiological properties of cholinergic transmission in the interpeduncular nucleus (IPN), we used a transgenic mouse line in which the light-gated cation channel ChannelRhodopsin-2 is selectively expressed in cholinergic neurons. Cholinergic axonal terminals were activated by light pulses, and postsynaptic responses were recorded from IPN neurons. Surprisingly, brief photostimulation produces fast excitatory postsynaptic currents that are mediated by ionotropic glutamate receptors, suggesting wired transmission of glutamate. By contrast, tetanic photostimulation generates slow inward currents that are largely mediated by nicotinic acetylcholine receptors, suggesting volume transmission of acetylcholine. Finally, vesicular transporters for glutamate and acetylcholine are coexpressed on the same axonal terminals in the IPN. These results strongly suggest that adult brain "cholinergic" neurons can corelease glutamate and acetylcholine, but these two neurotransmitters activate postsynaptic neurons via different transmission modes.
机译：乙酰胆碱是一种重要的神经递质，而脑间双突投射是大脑中的主要胆碱能途径。为了研究胆小神经节间核（IPN）中胆碱能传递的生理特性，我们使用了一种转基因小鼠品系，其中光门控阳离子通道ChannelRhodopsin-2在胆碱能神经元中选择性表达。胆碱能轴突末端被光脉冲激活，并从IPN神经元记录突触后反应。出人意料的是，短暂的光刺激会产生由离子型谷氨酸受体介导的快速兴奋性突触后电流，表明谷氨酸的有线传播。相比之下，强直性光刺激产生缓慢的内向电流，该电流主要由烟碱乙酰胆碱受体介导，表明乙酰胆碱的体积传递。最后，谷氨酸和乙酰胆碱的囊泡转运蛋白在IPN的同一轴突末端共表达。这些结果强烈表明，成人大脑“胆碱能”神经元可以共释放谷氨酸和乙酰胆碱，但是这两种神经递质通过不同的传输方式激活突触后神经元。

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