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首页> 外文期刊>Neuron >Dap160/intersectin acts as a stabilizing scaffold required for synaptic development and vesicle endocytosis.
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Dap160/intersectin acts as a stabilizing scaffold required for synaptic development and vesicle endocytosis.

机译：Dap160 / intersectin充当突触发育和囊泡内吞作用所需的稳定支架。

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摘要

We describe the isolation of mutations in dynamin-associated protein 160 kDa (dap160), the Drosophila homolog of intersectin, a putative adaptor for proteins involved in endocytosis, cytoskeletal regulation, and signaling. We show that partial loss-of-function mutants display temperature-sensitive (ts) paralysis, whereas null mutants show ts defects in endocytosis. Loss-of-function mutants exhibit bouton overgrowth at larval neuromuscular junctions (NMJs), but evoked neurotransmission is normal. Mutant NMJs show a mild endocytic defect at 22 degrees C, which is strongly enhanced at 34 degrees C. The levels of dynamin, synaptojanin and endophilin are severely reduced in dap160 mutant NMJs, suggesting that Dap160 serves to stabilize an endocytic macromolecular complex. Electron microscopy reveals fewer vesicles, aberrant large vesicles, and an accumulation of endocytic intermediates at active and periactive zones in mutant terminals. Our data suggest that Dap160, like dynamin, is involved in synaptic vesicle retrieval at active and periactive zones.
机译：我们描述了与动力蛋白相关的蛋白质160 kDa（dap160），果蝇intersectin的果蝇同源物，参与胞吞作用，细胞骨架调节和信号传导的蛋白质的假定适配器的突变体的分离。我们表明，部分功能丧失的突变体显示温度敏感（ts）瘫痪，而无效突变体在胞吞作用中显示ts缺陷。功能丧失的突变体在幼虫神经肌肉接头（NMJ）处显示肉豆蔻过度生长，但诱发的神经传递是正常的。突变的NMJ在22°C时显示出轻度的内吞缺陷，在34°C时会明显增强。在dap160突变的NMJ中，dynamin，突触素和内生蛋白的水平显着降低，这表明Dap160可以稳定内吞的大分子复合物。电子显微镜显示较少的囊泡，异常的大囊泡，并且在突变末端的活性区和活性区中积累了内吞性中间体。我们的数据表明，Dap160像动力蛋白一样，参与活动区和活动区的突触小泡修复。

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