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首页> 外文期刊>Neuron >Single-cell transcriptional analysis of neuronal progenitors.
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Single-cell transcriptional analysis of neuronal progenitors.

机译：神经元祖细胞的单细胞转录分析。

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摘要

The extraordinary cellular heterogeneity of the mammalian nervous system has largely hindered the molecular analysis of neuronal identity and diversity. In order to uncover mechanisms involved in neuronal differentiation and diversification, we have monitored the expression profiles of individual neurons and progenitor cells collected from dissociated tissue or captured from intact slices. We demonstrate that this technique provides a sensitive and reproducible representation of the single-cell transcriptome. In the olfactory system, hundreds of transcriptional differences were identified between olfactory progenitors and mature sensory neurons, enabling us to define the large variety of signaling pathways expressed by individual progenitors at a precise developmental stage. Finally, we show that regional differences in gene expression can be predicted from transcriptional analysis of single neuronal precursors isolated by laser capture from defined areas of the developing brain.
机译：哺乳动物神经系统的细胞异质性极大地阻碍了对神经元同一性和多样性的分子分析。为了揭示参与神经元分化和多样化的机制，我们已经监测了从分离组织中收集或从完整切片中捕获的单个神经元和祖细胞的表达谱。我们证明了这种技术提供了一种敏感和可再现的单细胞转录组的表示。在嗅觉系统中，在嗅觉祖细胞和成熟的感觉神经元之间发现了数百种转录差异，这使我们能够定义个体祖细胞在精确的发育阶段表达的多种信号通路。最后，我们表明可以通过对从发育中的大脑定义区域进行激光捕获而分离出的单个神经元前体进行转录分析来预测基因表达的区域差异。

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