...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neuron >Subcellular dynamics of type II PKA in neurons.
【24h】

Subcellular dynamics of type II PKA in neurons.

机译：神经元中II型PKA的亚细胞动力学。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Protein kinase A (PKA) plays multiple roles in neurons. The localization and specificity of PKA are largely controlled by A-kinase anchoring proteins (AKAPs). However, the dynamics of PKA in neurons and the roles of specific AKAPs are poorly understood. We imaged the distribution of type II PKA in hippocampal and cortical layer 2/3 pyramidal neurons in vitro and in vivo. PKA was concentrated in dendritic shafts compared to the soma, axons, and dendritic spines. This spatial distribution was imposed by the microtubule-binding protein MAP2, indicating that MAP2 is the dominant AKAP in neurons. Following cAMP elevation, catalytic subunits dissociated from the MAP2-tethered regulatory subunits and rapidly became enriched in nearby spines. The spatial gradient of type II PKA between dendritic shafts and spines was critical for the regulation of synaptic strength and long-term potentiation. Therefore, the localization and activity-dependent translocation of type II PKA are important determinants of PKA function.
机译：蛋白激酶A（PKA）在神经元中起多种作用。 PKA的定位和特异性在很大程度上由A激酶锚定蛋白（AKAP）控制。但是，人们对神经元中PKA的动力学和特定AKAP的作用了解得很少。我们在体外和体内对II型PKA在海马和皮层2/3锥体神经元中的分布进行了成像。与躯体，轴突和树突棘相比，PKA集中在树突柄中。这种空间分布是由微管结合蛋白MAP2强加的，表明MAP2是神经元中的主要AKAP。在cAMP升高后，催化亚基从与MAP2相连的调节亚基解离，并迅速富集在附近的棘中。树突状干和棘之间的II型PKA的空间梯度对于调节突触强度和长期增强至关重要。因此，II型PKA的定位和活性依赖性易位是PKA功能的重要决定因素。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号