Structure of GABARAP in two conformations: implications for GABA(A) receptor localization and tubulin binding.

Coyle JosephE; Qamar Seema; Rajashankar KanagalaghattaR; Nikolov DimitarB

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Structure of GABARAP in two conformations: implications for GABA(A) receptor localization and tubulin binding.

机译：GABARAP的结构有两种构象：对GABA（A）受体定位和微管蛋白结合的影响。

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GABARAP recognizes and binds the gamma2 subunit of the GABA(A) receptor, interacts with microtubules and the N-ethyl maleimide sensitive factor, and is proposed to function in GABA(A) receptor trafficking and postsynaptic localization. We have determined the crystal structure of human GABARAP at 1.6 A resolution. The structure comprises an N-terminal helical subdomain and a ubiquitin-like C-terminal domain. Structure-based mutational analysis demonstrates that the N-terminal subdomain is responsible for tubulin binding while the C-terminal domain contains the binding site for the GABA(A). A second GABARAP crystal form was determined at 1.9 A resolution and documents that GABARAP can self-associate in a head-to-tail manner. The structural details of this oligomerization reveal how GABARAP can both promote tubulin polymerization and facilitate GABA(A) receptor clustering.

机译：GABARAP识别并结合GABA（A）受体的gamma2亚基，与微管和N-乙基马来酰亚胺敏感因子相互作用，并被提议在GABA（A）受体运输和突触后定位中发挥作用。我们确定了1.6 A分辨率的人GABARAP的晶体结构。该结构包括N端螺旋亚结构域和泛素样C端结构域。基于结构的突变分析表明，N末端亚结构域负责微管蛋白结合，而C末端结构域包含GABA（A）的结合位点。第二个GABARAP晶型是在1.9 A分辨率下确定的，并记录了GABARAP可以从头到尾的方式自缔合。这种低聚的结构细节揭示了GABARAP如何既促进微管蛋白聚合又促进GABA（A）受体聚类。

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《Neuron》 |2002年第1期|共12页
作者
Coyle JosephE; Qamar Seema; Rajashankar KanagalaghattaR; Nikolov DimitarB;
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正文语种 eng
中图分类神经生理学;
关键词
Microtubule-Associated Proteins; Microtubules; Neural Inhibition; Protein Transport physiology; 微管相关蛋白质类; 微管; 神经抑制; 受体; GABA-A; 突触传递; 微管蛋白;

机译：Microtubule-Associated Proteins;Microtubules;Neural Inhibition;Protein Transport physiology;微管相关蛋白质类;微管;神经抑制;受体;GABA-A;突触传递;微管蛋白;

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1. Structure of GABARAP in two conformations: implications for GABA(A) receptor localization and tubulin binding. [J] . Coyle JosephE, Qamar Seema, Rajashankar KanagalaghattaR, Neuron . 2002,第1期

机译：GABARAP的结构有两种构象：对GABA（A）受体定位和微管蛋白结合的影响。
2. Conformational analysis of N,N-disubstituted-1,4-diazepane orexin receptor antagonists and implications for receptor binding. [J] . Cox CD, McGaughey GB, Bogusky MJ Bioorganic and Medicinal Chemistry Letters . 2009,第11期

机译：N，N-二取代-1,4-二氮杂ororexin受体拮抗剂的构象分析及其对受体结合的影响。
3. Immunohistochemical localization of GABAA receptors in comparison with GABA-immunoreactive structures in the nucleus tractus solitarii of the rat. [J] . Terai K, Tooyama I, Kimura H Neuroscience: An International Journal under the Editorial Direction of IBRO . 1998,第3期

机译：GABAA受体的免疫组织化学定位与大鼠孤束核中的GABA免疫反应结构相比。
4. Application of bioinformatics algorithms to define the most important protein features contribute to GABA receptors diversity GABA receptors' diversity, bioinformatic applications [C] . Ebrahimi Mansour, Ebrahimie Esmaeil 2nd International Conference on Chemical, Biological and Environmental Engineering . 2010

机译：应用生物信息学算法定义最重要的蛋白质特征有助于GABA受体多样性GABA受体的多样性，生物信息学应用
5. Regulation of allosteric modulation of GABA(a) receptor function: Interaction between recombinant GABA(A) receptors and the novel anticonvulsant drug topiramate. [D] . Simeone, Timothy Anthony, Jr. 2003

机译：调节GABA（a）受体功能的变构调节：重组GABA（A）受体与新型抗惊厥药托吡酯之间的相互作用。
6. Crystal structure of the GABAA-receptor-associated protein GABARAP [O] . Vassiliy N. Bavro, Maria Sola, Andreas Bracher, 2002

机译：GABAA受体相关蛋白GABARAP的晶体结构
7. Structure of GABARAP in Two Conformations Implications for GABAA Receptor Localization and Tubulin Binding [O] . Coyle Joseph E., Qamar Seema, Rajashankar Kanagalaghatta R., 2002

机译：GABARAP的结构对GABAA受体定位和微管蛋白结合的两个构想

Structure of GABARAP in two conformations: implications for GABA(A) receptor localization and tubulin binding.

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