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Auxin-cytokinin interaction and variations in their metabolic products in the regulation of organogenesis in two Eucomis species

机译：生长素-细胞分裂素相互作用及其代谢产物在调节两个杜仲属器官发生中的变化

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In the current study, we evaluated the effect of alpha-naphthaleneacetic acid (NAA) individually or in combination with different cytokinins (CKs) including benzyladenine (BA), meta-topolin (mT) and isopentenyladenine (iP) on organogenesis, auxin and CK content in Eucomis autumnalis subspecies autumnalis (EA) and Eucomis zambesiaca (EZ). These species were used as model plants due to their ornamental and medicinal properties. Three leaf explants were inoculated in screw-cap jars containing 30 mL Murashige and Skoog (MS) media supplemented with 5 mM NAA alone or in combination with 5 mu M CK (BA, mT or iP). After 10 weeks (EA) or 15 weeks (EZ), parameters including shoot and root growth as well as plant fresh weight were recorded. For analysis of auxin and CK content, whole plantlets were harvested, pooled and freeze-dried for the different treatments. In both species, shoot and root proliferation as well as plant biomass were generally higher when NAA was combined with the individual CK than in NAA or CK treatment. The highest concentration of indole-3-acetic acid (IAA, 619 pmol g(-1) DW) and 2-oxindole-3-acetic acid (OxIAA, 2381 pmol g(-1) DW) were observed in EA-treated with NAA alone while mT treatment (without NAA) had the most abundant indole-3-acetyl-L-aspartic acid (IAAsp, 904 and 582 pmol g(-1) DW for EA and EZ, respectively) in both species. A significant concentration of total endogenous CK accumulated in both Eucomis regenerants from mT and mT + NAA when compared to the other treatments. The majority of the detected CKs were of the aromatic CK-type, mainly free bases. The potential physiological roles of these quantified phytohormones in relation to the observed morphological responses are discussed. (C) 2016 Elsevier B.V. All rights reserved.
机译：在当前的研究中，我们评估了α-萘乙酸（NAA）单独或与不同细胞分裂素（CKs）包括苄基腺嘌呤（BA），间-topolin（mT）和异戊烯基腺嘌呤（iP）联合对器官发生，生长素和CK的作用。秋季杜仲（EA）和杜仲（EZ）的含量。这些物种由于其观赏和药用特性而被用作模型植物。将三片外植体接种在装有30 mL Murashige和Skoog（MS）培养基的螺帽瓶中，该培养基单独添加5 mM NAA或与5μM CK（BA，mT或iP）组合使用。在10周（EA）或15周（EZ）之后，记录包括枝条和根部生长以及植物鲜重在内的参数。为了分析植物生长素和CK的含量，收获全株苗，合并并冻干以用于不同处理。在两个物种中，NAA与单独的CK联合使用时，芽和根的增殖以及植物生物量通常高于NAA或CK处理。在EA处理的EA中，观察到最高浓度的吲哚-3-乙酸（IAA，619 pmol g（-1）DW）和2-羟吲哚-3-乙酸（OxIAA，2381 pmol g（-1）DW）。单独使用NAA而进行mT处理（不使用NAA）在两个物种中都具有最丰富的吲哚-3-乙酰基-L-天冬氨酸（IAAsp，EA和EZ分别为904和582 pmol g（-1）DW）。与其他处理相比，来自mT和mT + NAA的杜仲再生剂中积累的总内源性CK浓度显着升高。大部分检测到的CK是芳香CK型的，主要是游离碱。这些量化的植物激素与观察到的形态反应有关的潜在生理作用进行了讨论。 （C）2016 Elsevier B.V.保留所有权利。

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