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首页> 外文期刊>New biotechnology >Indole modifies the central carbon flux in the anaerobic metabolism of Escherichia coli: application to the production of hydrogen and other metabolites
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Indole modifies the central carbon flux in the anaerobic metabolism of Escherichia coli: application to the production of hydrogen and other metabolites

机译：吲哚修饰大肠杆菌厌氧代谢的中心碳通量：在生产氢气和其他代谢物中的应用

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Indole is a bicyclic signaling molecule with effects on both eukaryotic and prokaryotic cells. The majority of studies of indole action have been performed with bacteria cultured under aerobic conditions and little information is available about its effects under anaerobic conditions. Here the effect of the indole on anaerobic metabolism of Escherichia coli WDHL was studied. Indole in the range 0.5-8 mM was added to the culture medium and cell growth, hydrogen and metabolite production were compared to cultures lacking indole. Results showed that while 8 mM indole abolished growth completely, 4 mM indole had a partial bacteriostatic effect and the maximum optical density of the culture decreased by 44% compared to the control cultures. In addition, 4 mM indole had an important effect on anaerobic metabolism. Hydrogen production increased from 650 +/- 115 to 1137 +/- 343 mL H-2/L, and hydrogen yield increased from 0.45 +/- 0.1 to 0.94 +/- 0.34 mol H-2/mol glucose, compared to the control culture. Carbon flux was also affected and the composition of the final by-products changed. Lactate (41 mM) was the main metabolite in the control cultures, whereas ethanol (56.2 mM) and acetate (41.2 mM) were the main metabolites in the cultures with 2 mM indole. We conclude that the supplementation of E. coli cultures with exogenous indole is a simple and novel strategy to improve the production of hydrogen as well as other metabolites such as ethanol used as biofuels. (C) 2016 Elsevier B.V. All rights reserved.
机译：吲哚是一种对真核和原核细胞都有影响的双环信号分子。吲哚作用的大多数研究是在有氧条件下培养的细菌进行的，关于其在厌氧条件下的作用的信息很少。在这里研究了吲哚对大肠杆菌WDHL厌氧代谢的影响。将0.5-8 mM范围内的吲哚添加到培养基中，并将细胞生长，氢和代谢产物的生产与缺乏吲哚的培养物进行比较。结果显示，尽管8 mM吲哚完全消除了生长，但4 mM吲哚具有部分抑菌作用，与对照培养相比，培养物的最大光密度降低了44％。另外，4 mM吲哚对厌氧代谢有重要作用。与对照相比，产氢量从650 +/- 115增至1137 +/- 343 mL H-2 / L，氢产率从0.45 +/- 0.1增至0.94 +/- 0.34 mol H-2 / mol葡萄糖文化。碳通量也受到影响，最终副产物的组成发生了变化。乳酸（41 mM）是对照培养物中的主要代谢产物，而乙醇（56.2 mM）和乙酸盐（41.2 mM）是含2 mM吲哚的培养物中的主要代谢产物。我们得出结论，用外源吲哚补充大肠杆菌培养物是一种简单新颖的策略，可以提高氢气以及其他代谢产物（例如用作生物燃料的乙醇）的生产。 （C）2016 Elsevier B.V.保留所有权利。

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