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首页> 外文期刊>New biotechnology >A comparative study of solvent-assisted pretreatment of biodiesel derived crude glycerol on growth and 1,3-propanediol production from Citrobacter freundii
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A comparative study of solvent-assisted pretreatment of biodiesel derived crude glycerol on growth and 1,3-propanediol production from Citrobacter freundii

机译：溶剂辅助预处理生物柴油衍生甘油对弗氏柠檬酸杆菌生长和生产1,3-丙二醇的比较研究

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摘要

The rapidly growing biodiesel industry has created a scenario, where it is both important and challenging to deal with the enormous amount of crude glycerol generated as an inherent by-product. With every 100 gallons of biodiesel produced, 5–10 gallons of the crude glycerol is left behind containing several impurities which makes its disposal difficult. The objective of the present investigation was to evaluate the impact of biodiesel-derived crude glycerol upon microbial growth and production of 1,3- propanediol by Citrobacter freundii. Five different grades of crude glycerol (obtained from biodiesel preparation using jatropha, soybean, sunflower, rice bran and linseed oils) were used. Crude glycerol caused significant inhibition of microbial growth and subsequently 1,3-propanediol production as compared to pure glycerol. Therefore, a process was developed for the treatment of crude glycerol using solvents before fermentation wherein four different non-polar solvents were examined yielding different grades of pretreated glycerol. Subsequently, the potential toxic effects of pretreated glycerol on the growth and 1,3-propanediol production by C. freundii was evaluated. In case of petroleum ethertreated crude glycerol obtained from jatropha & linseed and hexane-treated crude glycerol obtained from rice bran, the yields obtained were comparable to the pure glycerol. Similarly, soybean-derived glycerol gave comparable results after treatment with either hexane or petroleum ether.
机译：快速发展的生物柴油行业创造了一个场景，其中处理作为固有副产物而产生的大量粗甘油既重要又具有挑战性。每生产100加仑生物柴油，就会留下5-10加仑的粗甘油，其中含有几种杂质，这使其难以处理。本研究的目的是评估生物柴油衍生的粗甘油对弗氏柠檬酸杆菌对微生物生长和1,3-丙二醇生产的影响。使用了五种不同等级的粗甘油（使用麻疯树油，大豆，向日葵，米糠和亚麻籽油从生物柴油制备中获得）。与纯甘油相比，粗甘油引起微生物生长的显着抑制和随后的1,3-丙二醇生成。因此，开发了一种在发酵前使用溶剂处理粗制甘油的方法，其中检查了四种不同的非极性溶剂，产生了不同等级的预处理甘油。随后，评估了预处理的甘油对弗氏梭菌的生长和1,3-丙二醇生产的潜在毒性作用。对于从麻疯树和亚麻子获得的石油醚处理的粗甘油和从米糠获得的己烷处理的粗甘油，其收率可与纯甘油相媲美。同样，大豆衍生的甘油在用己烷或石油醚处理后也得到了可比的结果。

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