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Improving lipid production from bagasse hydrolysate with Trichosporon fermentans by response surface methodology

机译：响应面法提高曲霉菌发酵物中蔗渣水解产物的脂质产量

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摘要

Oleaginous yeast Trichosporon fermentans was proved to be able to use sulphuric acid-treated sugar cane bagasse hydrolysate as substrate to grow and accumulate lipid. Activated charcoal was shown as effective as the more expensive resin Amberlite XAD-4 for removing the inhibitors from the hydrolysate. To further improve the lipid production, response surface methodology (RSM) was used and a 3-level 4-factor Box–Behnken design was adopted to evaluate the effects of C/N ratio, inoculum concentration, initial pH and fermentation time on the cell growth and lipid accumulation of T. fermentans. Under the optimum conditions (C/N ratio 165, inoculum concentration 11%, initial pH 7.6 and fermentation time 9 days), a lipid concentration of 15.8 g/L, which is quite close to the predicted value of 15.6 g/L, could be achieved after cultivation of T. fermentans at 25 8C on the pretreated bagasse hydrolysate and the corresponding lipid coefficient (lipid yield per mass of sugar, %) was 14.2. These represent a 32.8% improvement in the lipid concentration and a 21.4% increase in the lipid coefficient compared with the original values before optimization (11.9 g/L and 11.7). This work further demonstrates that T. fermentans is a promising strain for lipid production and thus biodiesel preparation from abundant and inexpensive lignocellulosic materials.
机译：事实证明，产油酵母曲霉孢子发酵剂能够使用硫酸处理的甘蔗渣蔗糖水解物作为底物来生长和积累脂质。活性炭显示出与更昂贵的树脂Amberlite XAD-4相同的有效作用，可从水解产物中除去抑制剂。为了进一步提高脂质产量，使用了响应表面方法（RSM），并采用了3级4因子Box–Behnken设计来评估C / N比，接种量，初始pH和发酵时间对细胞的影响发酵酵母的生长和脂质积累在最佳条件下（C / N比165，接种物浓度11％，初始pH 7.6和发酵时间9天），脂质浓度15.8 g / L可以非常接近预测值15.6 g / L。在发酵的甘蔗渣水解产物上于25 8℃培养发酵发酵单胞菌后，可得到相应的脂质系数（每糖质量的脂质产率，％）为14.2。与优化前的原始值（11.9 g / L和11.7）相比，这些代表脂质浓度提高了32.8％，脂质系数提高了21.4％。这项工作进一步证明，发酵曲霉是用于脂质生产的有希望的菌株，因此是从丰富而廉价的木质纤维素材料制备生物柴油的菌株。

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