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首页> 外文期刊>Materials science & engineering, C. Materials for Biogical applications >In vitro and in vivo evaluation of a new nanocomposite, containing high density polyethylene, tricalcium phosphate, hydroxyapatite, and magnesium oxide nanoparticles
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In vitro and in vivo evaluation of a new nanocomposite, containing high density polyethylene, tricalcium phosphate, hydroxyapatite, and magnesium oxide nanoparticles

机译：新型纳米复合材料的体外和体内评估，其中包含高密度聚乙烯，磷酸三钙，羟基磷灰石和氧化镁纳米颗粒

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摘要

In this study, a new nanocomposite, which contained high density polyethylene (HDPE), tricalcium phosphate (Ca_3(PO_4)_2) nanoparticles (TCP NPs), hydroxyapatite nanoparticles (HA NPs), and magnesium oxide nanoparticles (MgO NPs) was prepared. As in vitro experiment, human osteoblasts (HOB) cells were exposed to pristine HDPE and its nanocomposite for a period of 1, 4, and 7 days at 37 °C, and then different assays were carried out, including osteoblast cell proliferation, Trypan blue staining, cell viability, alkaline phosphatase (ALP), and cell adhesion. Antibacterial property of pristine HDPE and its nanocomposite was evaluated, and also their mechanical propertieswere measured after 2 and 4 months. As in vivo experiment, pristine HDPE and its nanocomposite were separately implanted on calvarium bone of rabbits, and tissue inflammation and osteogenesis were investigated after 2, 4, and 6 months. In case of HOB cells treated with HDPE or nanocomposite, as incubation time was increased, cell proliferation, live/dead ratio, and cell viability were decreased. But, the ALP activity and cell adhesion of HOB cells which treated with nanocomposite were raised after increase of incubation time. This study demonstrated that although the mechanical properties of nanocomposite were similar to HDPE sheet, but their antibacterial property was not similar. The in vivo experiment showed that both pristine HDPE and its nanocomposite had same inflammation responses. Interestingly, osteogenesis was observed after 2 months at boneanocomposite interface, and was highly increased after 4 and 6 months. It must be noted that such pattern was not seen at bone/HDPE interface.
机译：在这项研究中，制备了一种新的纳米复合材料，其中包含高密度聚乙烯（HDPE），磷酸三钙（Ca_3（PO_4）_2）纳米颗粒（TCP NPs），羟基磷灰石纳米颗粒（HA NPs）和氧化镁纳米颗粒（MgO NPs）。作为体外实验，将人类成骨细胞（HOB）在37°C的原始HDPE及其纳米复合材料中暴露1、4和7天，然后进行不同的测定，包括成骨细胞增殖，锥虫蓝染色，细胞活力，碱性磷酸酶（ALP）和细胞粘附。评估了原始HDPE及其纳米复合材料的抗菌性能，并在2和4个月后测量了它们的机械性能。作为体内实验，将原始的HDPE及其纳米复合材料分别植入兔的颅骨，并在2、4和6个月后研究组织炎症和成骨作用。对于用HDPE或纳米复合材料处理的HOB细胞，随着孵育时间的增加，细胞增殖，活/死比和细胞生存力都会降低。但是，随着孵育时间的延长，用纳米复合材料处理的HOB细胞的ALP活性和细胞粘附性增加。这项研究表明，尽管纳米复合材料的力学性能与HDPE片材相似，但其抗菌性能却不相似。体内实验表明原始的HDPE及其纳米复合材料具有相同的炎症反应。有趣的是，在骨/纳米复合材料界面2个月后观察到成骨现象，并在4个月和6个月后显着增加。必须注意的是，这种模式在骨骼/ HDPE界面上看不到。

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