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首页> 外文期刊>New horizons in translational medicine. >Efficacy of Pleurotus ostreatus (Jacq. Ex Fr.) P.kumm. on 7,12-dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats
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Efficacy of Pleurotus ostreatus (Jacq. Ex Fr.) P.kumm. on 7,12-dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats

机译：平菇的功效。对7,12-二甲基苯并（a）蒽诱导的雌性Sprague-Dawley大鼠乳腺癌致癌作用

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摘要

Neoplastic growth of the breast is the most common malignancy in women worldwide and its incidence has increased in most countries. In this study, we have evaluated the efficacy of Pleurotus ostreatus (Jacq. Ex Fr.) P.kumm. (P. ostreatus) an edible mushroom on modulating levels of xenobiotic metabolizing enzymes, hormonal status of estrogen and progesterone receptor (ER/PR), protein expressions and histopathological analysis in 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis using a rat model. DMBA was induced by single subcutaneous injection at a dosage of 25 mg in 1 mL vehicle. The ethanolic extract of P. ostreatus (POEet) was administered orally at a concentration of 600 mg/kg bwt as pre- and post-initiation stage of treatment throughout the experimental period which was also compared with standard tamoxifen (TAM) (10 mg/kg bwt). At the end of 16 weeks, our results showed the elevated phase I and depleted phase II metabolizing enzymes, over expression of (ER/PR) and the expression pattern of the proteins such as fas, fasL, caspase 3, caspase 8, caspase 9, Bax were found to be down regulated whereas p53, Bcl2, cox-2 and cyclin D1 were markedly upregulated in DMBA-induced Sprague-Dawley rats, which were significantly reversed on P. ostreatus administration. Moreover, pre-treatment with P. ostreatus showed improved response when compared to that of posttreatment. Based on scientific appraisal, we conclude that the dietary consumption of P. ostreatus might offer maximum protection against DMBA-induced mammary carcinogenesis and improving human health if used as a regular basis. Focal points ? Benchside ○ The potential of Pleurotus ostreatus on DMBA induced rat mammary carcinogenesis were determined by the analysis of xenobiotic metabolizing enzymes, hormonal status of ER/PR, as well as the expression of protein using western blotting techniques and histological examination of liver and mammary tissues.? Bedside ○ The pharmaceutical potential of P. ostreatus are analysed for is markedly available, edible one with high protein as well as fiber with low fat content and cost effective for patient convenience.? Industry ○ Mushrooms are considered as a nutraceutical functional food which can contain enriched prolific produced of novel “mycochemicals” responsible for the human health potential on various diseases and malignancies.? Community ○ Standardization and refinement of mycochemical from mushrooms were help to develop the new novel active compounds which contribute for better health and also help to reduce disease burden.? Regulatory agencies ○ The P. ostreatus contain several bioactive compounds which might be the key factor for the anticancer effect. This will need to be taken for labeling and pattering the compounds and tested in clinical trials.
机译：乳腺癌的赘生性生长是全世界女性最常见的恶性肿瘤，在大多数国家，其发病率均有所上升。在这项研究中，我们评估了平菇（Pacuro ostreatus）（Jacq。Ex Fr.）P.kumm的功效。 （P. ostreatus）一种可食用蘑菇，可调节7,12-二甲基苯并（a）蒽（DMBA）诱导的乳腺癌致癌作用中的异种生物代谢酶水平，雌激素和孕激素受体（ER / PR）的激素状态，蛋白质表达以及组织病理学分析使用老鼠模型。通过在1 mL溶媒中以25 mg剂量单次皮下注射诱导DMBA。在整个实验期间的治疗开始和治疗后阶段，以600 mg / kg bwt的浓度口服口服P. ostreatus乙醇提取物（POEet），并将其与标准他莫昔芬（TAM）（10 mg / kg kg bwt）。在16周结束时，我们的结果显示I期代谢酶和II期代谢酶减少，（ER / PR）的过度表达以及fas，fasL，caspase 3，caspase 8，caspase 9等蛋白质的表达模式在DMBA诱导的Sprague-Dawley大鼠中，发现Bax的表达下调，而p53，Bcl2，cox-2和cyclin D1显着上调，在服用P. ostreatus时，这种表达明显逆转。此外，与后处理相比，用平菇进行的预处理显示出改善的反应。根据科学评估，我们得出的结论是，如果定期食用平菇，饮食可以最大程度地防止DMBA诱导的乳腺癌致癌作用并改善人体健康。联络点？实验台○平菇对DMBA诱导的大鼠乳腺癌发生的潜力通过分析异源生物代谢酶，ER / PR的激素状态以及蛋白质的表达（通过蛋白质印迹技术以及肝和乳腺组织的组织学检查）来确定。 ？床头○分析了P. ostreatus的潜在药理，可以找到一种可食用的，高蛋白，脂肪含量低且成本低，方便患者的纤维。工业○蘑菇被认为是一种营养保健功能性食品，其中可能含有丰富的多产的新型“霉菌化学物质”，这些物质对人类在各种疾病和恶性肿瘤上的健康潜力具有重要意义。社区○蘑菇中真菌化学物质的标准化和提纯有助于开发新的新型活性化合物，这些化合物有助于改善健康状况，还有助于减轻疾病负担。监管机构○平菇含有几种生物活性化合物，这可能是抗癌作用的关键因素。这将需要用于标记和构图化合物并在临床试验中进行测试。

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