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首页> 外文期刊>New Journal of Chemistry >Fluorescent molecular nanocrystals anchored in sol-gel thin films: a label-free signalization function for biosensing applications
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Fluorescent molecular nanocrystals anchored in sol-gel thin films: a label-free signalization function for biosensing applications

机译：锚固在溶胶-凝胶薄膜中的荧光分子纳米晶体：用于生物传感应用的无标记信号功能

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摘要

A DNA sensor based on highly fluorescent and photostable molecular nanocrystals has been investigated. These nanocrystals are confined in a sol-gel thin film by a simple one-step process. After controlled dissolution of the sol-gel thin film layer-by-layer, nanocrystals emerge from the silicate matrix. Hairpin-shaped DNA fragments, functionalized by a probe molecule, were chemically bound to these nanocrystals. This grafting brings molecular probes close to the nanocrystal surface, resulting in quenching of the nanocrystal fluorescence by Forster Resonance Energy Transfer. After hybridization of the hairpin with their complementary DNA, they become unfolded, moving the probes away from the nanocrystals. This "turns on" the fluorescence of the nanocrystals and allows the hybridization detection of non-fluorescent DNA to be achieved. These first results on fluorescent nanocomposite coatings open up promising possibilities for making label-free generic sensors.
机译：已经研究了基于高度荧光和光稳定的分子纳米晶体的DNA传感器。通过简单的一步法将这些纳米晶体限制在溶胶-凝胶薄膜中。在控制溶胶-凝胶薄膜的逐层溶解之后，纳米晶体从硅酸盐基质中出现。通过探针分子功能化的发夹状DNA片段化学结合到这些纳米晶体上。该接枝使分子探针接近纳米晶体表面，从而通过福斯特共振能量转移使纳米晶体荧光猝灭。将发夹与其互补的DNA杂交后，它们会展开，将探针从纳米晶体上移开。这“打开”了纳米晶体的荧光，并允许进行非荧光DNA的杂交检测。荧光纳米复合材料涂层的这些最初结果为制造无标签的通用传感器打开了广阔的前景。

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