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首页> 外文期刊>Neuroscience: An International Journal under the Editorial Direction of IBRO >Role of peripheral mu-opioid receptors in inflammatory orofacial muscle pain.
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Role of peripheral mu-opioid receptors in inflammatory orofacial muscle pain.

机译：周围类阿片受体在发炎性口面部肌肉疼痛中的作用。

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The aims of this project were to investigate whether inflammation in the orofacial muscle alters mu opioid receptor (MOR) mRNA and protein expressions in trigeminal ganglia (TG), and to assess the contribution of peripheral MORs under acute and inflammatory muscle pain conditions. mRNA and protein levels for MOR were quantified by reverse-transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively, from the TG of naive rats, and compared with those from the rats treated with complete Freund's adjuvant (CFA) in the masseter. TG was found to express mRNA and protein for MOR, and CFA significantly up-regulated both MOR mRNA and protein by 3 days following the inflammation. The MOR protein up-regulation persisted to day 7 and returned to the baseline level by day 14. We then investigated whether peripheral application of a MOR agonist, D-Ala2, N-Me-Phe4, Gly5-ol-enkephalin acetate salt (DAMGO), attenuates masseter nociception induced by masseteric infusion of hypertonic saline (HS) in lightly anesthetized rats. DAMGO (1, 5, 10 microg) or vehicle was administered directly into the masseter 5-10 min prior to the HS infusion. The DAMGO effects were assessed on mean peak counts (MPC) and overall magnitude as calculated by the area under the curve (AUC) of the HS-evoked behavioral responses. Under this condition, only the highest dose of DAMGO (10 microg) significantly reduced MPC, which was prevented when H-D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH2 (CTAP), a selective MOR antagonist, was co-administered. DAMGO pre-treatment in the contralateral masseter did not attenuate MPC. The same doses of DAMGO administered into CFA-inflamed rats, however, produced a greater attenuation of both MPC and AUC of HS-evoked nocifensive responses. These results demonstrated that activation of peripheral MOR provides greater anti-nociception in inflamed muscle, and that the enhanced MOR effect can be partly explained by significant up-regulation of MOR expression in TG.
机译：该项目的目的是研究口面部肌肉的炎症是否会改变三叉神经节（TG）中的μ阿片受体（MOR）mRNA和蛋白表达，并评估急性和炎症性肌肉疼痛情况下外周MOR的贡献。分别通过逆转录-聚合酶链反应（RT-PCR）和Western印迹分别从幼稚大鼠的TG中定量测定MOR的mRNA和蛋白水平，并与接受完全弗氏佐剂（CFA）治疗的大鼠的相比。咬肌。发现TG表达MOR的mRNA和蛋白，并且发炎后3天，CFA显着上调MOR mRNA和蛋白。 MOR蛋白的上调一直持续到第7天，并在第14天恢复到基线水平。然后，我们研究了MOR激动剂，D-Ala2，N-Me-Phe4，Gly5-ol-脑啡肽乙酸盐（DAMGO）是否外围应用），可减轻轻度麻醉大鼠高渗盐水（HS）的大剂量输注引起的咬肌痛觉。在HS输注前5-10分钟，将DAMGO（1、5、10微克）或溶媒直接施入咬肌。评估DAMGO的作用是通过HS诱发的行为反应曲线下面积（AUC）计算出的平均峰值数（MPC）和总体强度。在这种情况下，只有最高剂量的DAMGO（10微克）才能显着降低MPC，而HD-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH2（CTAP）（选择性MOR）则可以避免拮抗剂，共同给药。在对侧咬肌中进行DAMGO预处理不会减弱MPC。但是，在接受CFA的大鼠中施用相同剂量的DAMGO，会使HS诱发的伤害性反应的MPC和AUC减弱更大。这些结果表明，外周MOR的活化在发炎的肌肉中提供了更大的抗伤害感受性，并且增强的MOR作用可以部分由TG中MOR表达的显着上调来解释。

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