Comparative Plasma Membrane-Associated Proteomics of Immortalized Human Hepatocytes

Lan-Tu Gou; Ai-Ping Tong; Li-Juan Chen

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Comparative Plasma Membrane-Associated Proteomics of Immortalized Human Hepatocytes

机译：永生化人类肝细胞的比较血浆膜相关蛋白质组学

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This work was initiated with the purpose of purifying and identifying differentially expressed plasma membrane-associated proteins between human liver cancer cell line HepG2 and normal liver cell line L02.The combined strategy of sucrose density gradient centrifugation and subsequent phase partition was applied to obtain high-purity proteins of plasma membrane.Two-dimensional gel electrophoresis revealed the differential protein profile between the two cell lines.A total of 13 plasma membrane-associated proteins containing 10 up-regulated proteins and three down-regulated proteins in HepG2 cells were successfully identified by MALDI-Q-TOF mass spectrometry;they participate in multiple biological functions such as adhesion,proliferation,apoptosis,and signal transduction.The identified proteins could provide helpful reference in clinical investigations on potential candidates for diagnosis and therapy of liver cancer.

机译：这项工作的目的是纯化和鉴定人肝癌细胞系HepG2和正常肝细胞系L02之间差异表达的质膜相关蛋白。采用蔗糖密度梯度离心和随后的相分配相结合的策略来获得高二维凝胶电泳显示了两个细胞系之间的差异蛋白质谱。通过HepG2细胞成功鉴定了总共13种质膜相关蛋白，其中包含10种上调蛋白和3种下调蛋白。 MALDI-Q-TOF质谱法具有多种生物学功能，如黏附，增殖，凋亡和信号转导。这些鉴定出的蛋白可以为肝癌的诊断和治疗潜在候选物的临床研究提供参考。

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《Biochemistry》 |2008年第11期|共7页
作者
Lan-Tu Gou; Ai-Ping Tong; Li-Juan Chen;
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正文语种 eng
中图分类生物化学;
关键词
plasma membrane; liver cancer; sucrose density gradient centrifugation; two-dimensional gel electrophoresis; proteomics; MALDI-Q-TOF;

机译：细胞膜;肝癌;蔗糖密度梯度离心;二维凝胶电泳;蛋白质组学;MALDI-Q-TOF;

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1. Comparative Plasma Membrane-Associated Proteomics of Immortalized Human Hepatocytes [J] . Lan-Tu Gou, Ai-Ping Tong, Li-Juan Chen Biochemistry . 2008,第11期

机译：永生化人类肝细胞的比较血浆膜相关蛋白质组学
2. Isobaric Tags for Relative and Absolute Quantification-based Comparative Proteomics Reveals the Features of Plasma Membrane-Associated Proteomes of Pollen Grains and Pollen Tubes from Lilium davidii [J] . Bing Han, Sixue Chen, Shaojun Dai, 植物学报（英文版） . 2010,第012期

机译：基于相对定量和绝对定量的比较蛋白质组学的等压标记揭示了质膜的蛋白质膜相关蛋白的特征，这些蛋白与大卫百合的花粉粒和花粉管有关。
3. Isobaric Tags for Relative and Absolute Quantificationbased Comparative Proteomics Reveals the Features of Plasma Membrane-Associated Proteomes of Pollen Grains and Pollen Tubes from Lilium davidii [J] . Bing Han, Sixue Chen, Shaojun Dai, 植物学报：英文版 . 2010,第012期

机译：基于相对定量和绝对定量的比较蛋白质组学的等压标记揭示了百合百合花粉粒和花粉管的质膜相关蛋白质组学特征
4. A rapid label-free proteomics survey of immortalization in cultured human breast epithelial cells [C] . Rui Miguel Mamede Branca, Virginia P. Pearce, Laszlo Prokai American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：无快速的无标签蛋白质组学调查培养人乳腺上皮细胞中的永生化
5. Evaluation of two novel models to examine the human xenobiotic-response: An immortalized human hepatocyte cell line and a 'humanized' pregnane X receptor mouse. [D] . Brobst, Dan E. 2006

机译：评估两种新型模型以检查人源生物反应：永生化的人肝细胞系和“人化的”孕烷X受体小鼠。
6. Comparative Proteomics Reveals Novel Components at the Plasma Membrane of Differentiated HepaRG Cells and Different Distribution in Hepatocyte- and Biliary-Like Cells [O] . Catalina Petrareanu, Alina Macovei, Izabela Sokolowska, -1

机译：比较蛋白质组学揭示了已分化的HepaRG细胞的质膜上的新成分以及在肝细胞和胆道样细胞中的不同分布
7. Isobaric Tags for Relative and Absolute Quantification- based Comparative Proteomics Reveals the Features of Plasma Membrane-Associated Proteomes of Pollen Grains and Pollen Tubes from Lilium davidii [O] . Bing Han, Sixue Chen, Shaojun Dai, 2010

机译：基于相对和绝对的量化的比较蛋白质组学的阻碍了血浆膜和花粉管的特征来自Lilium Davidii的特征

Comparative Plasma Membrane-Associated Proteomics of Immortalized Human Hepatocytes

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