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首页> 外文期刊>Biochemistry >Cloning and Functional Identification of Two Novel BRCA1 Splicing Variants
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Cloning and Functional Identification of Two Novel BRCA1 Splicing Variants

机译：两个新的BRCA1剪接变体的克隆和功能鉴定

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BRCA1 is an important tumor suppressor gene associated with inherited breast and ovarian cancers.In this investigation,two novel BRCA1 splicing variants were cloned from breast cancer cell line ZR-75-30.These transcripts,named .MG47-PI21-A2-21 and BRCA1-A2-14,lacked most exons of full length BRCA1 gene,but maintained the original reading frame.We also demonstrated the presence of BRA1-PI21-DELTA 2-21 in several human cell lines.Expression of both variants fused with green fluorescent protein(GFP)showed that they targeted different subcellular compartments in the transfect-ed cells.Viability of the cells expressing both fusion proteins decreased notably compared with the viability of cells expressing only GFP.Fluorescence activated cell sorting assay confirmed that the overexpression of two splicing variants resulted in cell apoptosis.Taken together,the different subcellular localization and cell effects of two BRCA1 splicing variants imply that they can have different biological functions in breast cancer cells.Elucidating the functions of BRCAl splicing variants would help to understand the exact roles of the BRCA1 gene in tumor suppression.
机译：BRCA1是与遗传性乳腺癌和卵巢癌有关的重要肿瘤抑制基因。在这项研究中，从乳腺癌细胞系ZR-75-30中克隆了两个新型BRCA1剪接变体。这些转录本分别称为.MG47-PI21-A2-21和BRCA1-A2-14，缺乏全长BRCA1基因的大多数外显子，但保持了原有的阅读框架。我们还证明了BRA1-PI21-DELTA 2-21在几种人类细胞系中的存在。两种变体融合绿色荧光的表达蛋白（GFP）表明它们靶向转染的细胞中不同的亚细胞区室。表达两种融合蛋白的细胞的活力与仅表达GFP的细胞的活力相比显着降低。荧光激活细胞分选测定证实了两个剪接的过表达两个BRCA1剪接变体的亚细胞定位和细胞效应不同，这意味着它们可以具有不同的生物学功能。阐明BRCA1剪接变体的功能将有助于了解BRCA1基因在肿瘤抑制中的确切作用。

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