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首页> 外文期刊>North American Journal of Aquaculture >The Influence of Dietary Lysine on Yellow Perch Female Reproductive Performance and the Quality of Eggs
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The Influence of Dietary Lysine on Yellow Perch Female Reproductive Performance and the Quality of Eggs

机译:日粮赖氨酸对黄鲈雌性繁殖性能和蛋品质的影响

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The objectives of this study were to (1) evaluate if dietary lysine (Lys) has an effect on the free amino acid (FAA) pool of Yellow Perch Perca flavescens eggs, and (2) determine how dietary Lys influences the reproductive performance and eggs fertilization rate (embryo survival) of female Yellow Perch. Two-year-old Yellow Perch of initial size of approximately 75 g were randomly distributed into six 400-L tanks at 32 +/- 1 fish per tank. This experiment included two wheat gluten-based diets in triplicate Lys-deficient ([-] Lys) and Lys-supplemented ([+]Lys; 2.23% in dry feed) diets. Females from the reference group were fed a commercial diet. Females from reference, (+) Lys, and (-) Lys groups were stripped and their eggs divided into 0.4-1.2-g portions and mixed with sperm (21.4 +/- 4.3 mu L) from either reference, (+) Lys, or (-) Lys males. The mean weight of Yellow Perch females and mean total weight of ovulated eggs were the greatest in the reference group compared with both (+) Lys and (-) Lys groups. There were no differences in the ratio of weight of eggs to female body weight as well as egg size among groups. There was no difference among treatments in the concentration of free amino acids except glutamic and aspartic acids in Yellow Perch eggs. There was significant effect of female dietary treatments on the egg fertilization rate averaged across all males. The higher fertilization rate was observed in the reference and (+) Lys groups compared with the (-) Lys group. The effect of female dietary treatment on the egg survival was also dependent on the dietary treatment of males.
机译:这项研究的目的是(1)评估赖氨酸(Lys)是否对黄鲈(Perca flavescens)卵的游离氨基酸(FAA)池有影响,以及(2)确定赖氨酸(Lys)如何影响生殖性能和卵黄鲈的受精率(胚胎存活率)。初始大小约为75 g的两岁黄鲈随机分布在六个400-L的水箱中,每条鱼32 +/- 1条鱼。该实验包括两种基于小麦麸质的饮食,一式三份缺乏Lys([-] Lys)和补充Lys([+] Lys;干饲料为2.23%)。参比组的女性接受商业饮食。剥去参照,(+)Lys和(-)Lys组的雌性,将卵分成0.4-1.2-g份,并与(+)Lys,(+)Lys的精子混合(21.4 +/- 4.3μL)。或(-)Lys男性。与(+)Lys和(-)Lys组相比,参考组的黄鲈雌性平均重量和排卵卵平均总重量最大。各组之间蛋重与女性体重的比例以及蛋大小没有差异。除黄鲈卵中的谷氨酸和天冬氨酸外,各处理之间的游离氨基酸浓度没有差异。女性饮食治疗对所有男性平均卵子受精率有显着影响。与(-)Lys组相比,参考和(+)Lys组的受精率更高。女性饮食治疗对卵子存活的影响还取决于男性的饮食治疗。

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