BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting - art. no. e118

Crowe ML; Rana D; Fraser F; Bancroft I; Trick M

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BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting - art. no. e118

机译：BACFinder：基于限制性指纹图谱的大插入基因组克隆的基因组定位-艺术。没有。 e118

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We have developed software that allows the prediction of the genomic location of a bacterial artificial chromosome (BAC) clone, or other large genomic clone, based on a simple restriction digest of the BAC. The mapping is performed by comparing the experimentally derived restriction digest of the BAC DNA with a virtual restriction digest of the whole genome sequence. Our trials indicate that this program identified the genomic regions represented by BAC clones with a degree of accuracy comparable to that of end-sequencing, but at considerably less cost. Although the program has been developed principally for use with Arabidopsis BACs, it should align large insert genomic clones to any fully sequenced genome.

机译：我们已经开发了一种软件，该软件可以基于BAC的简单限制性消化来预测细菌人工染色体（BAC）克隆或其他大型基因组克隆的基因组位置。通过比较实验得出的BAC DNA限制性酶切消化物和整个基因组序列的虚拟限制性酶切消化物来进行定位。我们的试验表明，该程序以与末端测序相当的准确度鉴定了BAC克隆代表的基因组区域，但成本却低得多。尽管该程序主要是为与拟南芥BAC一起使用而开发的，但它应将大插入基因组克隆与任何完全测序的基因组比对。

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《Nucleic Acids Research》 |2002年第21期|共1页
作者
Crowe ML; Rana D; Fraser F; Bancroft I; Trick M;
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正文语种 eng
中图分类细胞形态学;生物化学;
关键词
Cloning; Fragments; Vector; Gene; Dna;

机译：克隆;片段;载体;基因;DNA;

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专利

1. BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting - art. no. e118 [J] . Crowe ML, Rana D, Fraser F, Nucleic Acids Research . 2002,第21期

机译：BACFinder：基于限制性指纹图谱的大插入基因组克隆的基因组定位-艺术。没有。 e118
2. Genome physical mapping from large-insert clones by fingerprint analysis with capillary electrophoresis: a robust physical map of Penicillium chrysogenum - art. no. e50 [J] . Xu ZY, van den Berg MA, Scheuring C, Nucleic Acids Research . 2005,第5期

机译：大插入克隆的基因组物理图谱通过毛细管电泳指纹分析：产黄青霉的强大物理图谱-艺术。没有。 e50
3. Genomic representations using concatenates of Type IIB restriction endonuclease digestion fragments - art. no. e121 [J] . Tengs T, LaFramboise T, Den RB, Nucleic Acids Research . 2004,第15期

机译：使用IIB型限制性核酸内切酶消化片段串联体的基因组表征-art。没有。 e121
4. A model for comparing genomic restriction maps [C] . Platt, D.M., Dix, . 1995

机译：比较基因组限制性图谱的模型
5. Xanthomonas pathovars identification through a neural network-based genomic fingerprint classification system. [D] . Tuang, Fei Ni. 1998

机译：通过基于神经网络的基因组指纹分类系统识别黄单胞菌病。
6. BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting [O] . Mark L. Crowe, Debashis Rana, Fiona Fraser, 2002

机译：BACFinder：基于限制性指纹图谱的大插入基因组克隆的基因组定位
7. BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting [O] . Crowe, Mark L., Rana, Debashis, Fraser, Fiona, 2002

机译：BACFinder：基于限制性指纹图谱的大插入基因组克隆的基因组定位

BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting - art. no. e118

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