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首页> 外文期刊>Nucleic Acids Research >REQUIREMENTS FOR CLEAVAGE BY A MODIFIED RNASE P OF A SMALL MODEL SUBSTRATE
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REQUIREMENTS FOR CLEAVAGE BY A MODIFIED RNASE P OF A SMALL MODEL SUBSTRATE

机译：小模型底物的修饰RNASE P裂解的要求

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摘要

M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli, has been covalently linked at its 3' terminus to oligonucleotides (guide sequences) that guide the enzyme to target RNAs through hybridization with the target sequences. These constructs (M1GS RNAs) have been used to determine some minimal features of model substrates. As few as 3 bp on the 3' side of the site of cleavage in a substrate complex and 1 nt on the 5' side are required for cleavage to occur, The cytosines in the 3' terminal CCA sequence of the model substrates are important for cleavage efficiency but not cleavage site selection. A purine (base-paired or not) at the 3' side of the cleavage site is important both for cleavage site selection and efficiency, M1GS RNAs provide both a simple system for characterization of the reaction governed by M1 RNA and a tool for gene therapy.
机译：M1 RNA是大肠杆菌RNase P的催化RNA亚基，已在其3'端与寡核苷酸（引导序列）共价连接，该寡核苷酸可通过与靶序列杂交将酶引导至靶RNA。这些构建体（M1GS RNA）已用于确定模型底物的某些最小特征。在底物复合物的切割位点的3'侧需要少至3 bp，在5'侧需要1 nt才能发生切割。模型底物的3'末端CCA序列中的胞嘧啶对于切割效率，但不切割位点选择。裂解位点3'侧的嘌呤（是否有碱基配对）对于裂解位点的选择和效率都很重要，M1GS RNA既提供了表征M1 RNA的反应的简单系统，又提供了基因治疗的工具。

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