...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nucleic Acids Research >TRANSCRIPTION AND POLYADENYLATION IN A SHORT HUMAN INTERGENIC REGION
【24h】

TRANSCRIPTION AND POLYADENYLATION IN A SHORT HUMAN INTERGENIC REGION

机译：人类内源性短区域的转录和聚丙烯酰化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The poly(A) signal of the human Lamin B2 gene was previously shown to lie 600 bp upstream of the cap site of a gene of unknown function (ppv1). However, using RNase protection analysis, we show that ppv1 has two clusters of multiple initiation sites, so that the 5' cap site lies only similar to 280 nt downstream of the Lamin B2 poly(A) signal. We analysed nascent transcription across this unusually short intergenic region using nuclear run-on analysis of both the endogenous locus and of transiently transfected hybrid constructs. Surprisingly, transcription of the Lamin B2 gene does not appear to terminate prior to any of the mapped ppv1 start sites, although pausing of the elongating polymerase complexes is observed downstream of the Lamin B2 poly(A) signal. We suggest that this pausing may be sufficient to protect the downstream gene from transcriptional interference. Finally, we have also investigated the sequences required for efficient recognition of the Lamin B2 poly(A) signal. We show that sequences upstream of the AAUAAA element are required for full activity, which is an unusual feature of mammalian poly(A) signals.
机译：先前显示，人类Lamin B2基因的poly（A）信号位于功能未知基因（ppv1）的帽位点上游600 bp。但是，使用RNase保护分析，我们显示ppv1具有两个簇的多个起始位点，因此5'帽位仅位于Lamin B2 poly（A）信号下游的280 nt处。我们使用内源基因座和瞬时转染的杂交构建体的核运行分析分析了这个异常短的基因间区域的新生转录。出人意料的是，尽管在Lamin B2 poly（A）信号的下游观察到了延伸聚合酶复合物的暂停，但Lamin B2基因的转录似乎并未在任何映射的ppv1起始位点之前终止。我们建议这种暂停可能足以保护下游基因免受转录干扰。最后，我们还研究了有效识别Lamin B2 poly（A）信号所需的序列。我们表明，AAUAAA元素上游的序列是完整活动所必需的，这是哺乳动物poly（A）信号的不寻常特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号