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首页> 外文期刊>Nucleic Acids Research >Expression specificity of the mouse exonuclease 1 (mExo1) gene.
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Expression specificity of the mouse exonuclease 1 (mExo1) gene.

机译：小鼠核酸外切酶1（mExo1）基因的表达特异性。

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Genetic recombination involves either the homo-logous exchange of nearly identical chromosome regions or the direct alignment, annealing and ligation of processed DNA ends. These mechanisms are involved in repairing potentially lethal or mutagenic DNA damage and generating genetic diversity within the meiotic cell population and antibody repertoire. We report here the identification of a mouse gene, termed mExo1 for mouse exonuclease 1, which encodes a approximately 92 kDa protein that shares homology to proteins of the RAD2 nuclease family, most notably human 5' to 3' exonuclease Hex1/hExo1, yeast exonuclease 1 (Exo1) proteins and Drosophila melanogaster Tosca. The mExo1 gene maps to distal chromosome 1, consistent with the recent mapping of the orthologous HEX1 / hEXO1 gene to chromosome 1q42-q43. mExo1 is expressed prominently in testis, an area of active homologous recombination, and spleen, a prominent lymphoid tissue. An increased level of mExo1 mRNA was observed during a stage of testis development where cells that are actively involved in meiotic recombination arise first and represent a significant proportion of the germ cell population. Comparative evaluation of the expression patterns of the human and mouse genes, combined with previous biochemical and yeast genetic studies, indicate that the Exo1-like proteins are important contributors to chromosome processing during mammalian DNA repair and recombination.
机译：遗传重组涉及到几乎相同的染色体区域的同源交换或已加工DNA末端的直接比对，退火和连接。这些机制参与修复潜在的致死性或诱变性DNA损伤，并在减数分裂细胞群和抗体库中产生遗传多样性。我们在这里报告了一种小鼠基因的鉴定，该基因被称为小鼠核酸外切酶1的mExo1，其编码约92 kDa的蛋白质，该蛋白质与RAD2核酸酶家族的蛋白质具有同源性，最著名的是人类5'至3'核酸外切酶Hex1 / hExo1，酵母核酸外切酶1（Exo1）蛋白和果蝇Tosca。 mExo1基因映射到远端染色体1，与直系同源HEX1 / hEXO1基因最近映射到染色体1q42-q43一致。 mExo1在睾丸（活跃的同源重组区域）和脾脏（在淋巴样组织中）突出表达。在睾丸发育阶段观察到mExo1 mRNA的水平升高，在该阶段中，首先参与减数分裂重组的细胞首先出现，并代表了生殖细胞群的很大一部分。对人类和小鼠基因表达模式的比较评估，以及先前的生化和酵母遗传研究表明，Exo1样蛋白是哺乳动物DNA修复和重组过程中染色体加工的重要贡献者。

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