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首页> 外文期刊>Nucleosides, nucleotides and nucleic acids >A New Organic Dye-Based Staining for The Detection of Plant DNA in Agarose Gels
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A New Organic Dye-Based Staining for The Detection of Plant DNA in Agarose Gels

机译：一种用于检测琼脂糖凝胶中植物DNA的新型基于有机染料的染色

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Ethidium bromide (EtBr) is used to stain DNA in agarose gel electrophoresis, but this dye is mutagenic and carcinogenic. We investigated N-719, which is a visible, reliable and organic Ruthenium-based dye, and five fluorescent alternatives for staining plant DNA. For prestaining and poststaining, N-719, GelRed, and SYBR Safe stained both DNA and PCR product bands as clearly as EtBr. SYBR Green I, methylene blue, and crystal violet were effective for poststaining only. The organic dye N-719 stained DNA bands as sensitively and as clearly as EtBr. Consequently, organic dyes can be used as alternatives to EtBr in plant biotechnology studies.
机译：溴化乙锭（EtBr）用于在琼脂糖凝胶电泳中对DNA染色，但该染料具有诱变性和致癌性。我们研究了N-719，这是一种可见，可靠且基于有机钌的染料，另外还有五种荧光染料可用于植物DNA的染色。对于染色和后期染色，N-719，GelRed和SYBR Safe对DNA和PCR产物条带的染色与EtBr一样清晰。 SYBR Green I，亚甲基蓝和结晶紫仅对后染色有效。有机染料N-719与EtBr一样敏感和清晰地染色了DNA条带。因此，在植物生物技术研究中，有机染料可用作EtBr的替代品。

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