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首页> 外文期刊>Nucleus >Fission yeast Lem2 and Mani perform fundamental functions of the animal cell nuclear lamina
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Fission yeast Lem2 and Mani perform fundamental functions of the animal cell nuclear lamina

机译：裂变酵母Lem2和Mani发挥动物细胞核层的基本功能

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摘要

In animal cells the nuclear lamina, which consists of lamins and lamin-associated proteins, serves several functions: it provides a structural scaffold for the nuclear envelope and tethers proteins and heterochromatin to the nuclear periphery. In yeast, proteins and large heterochromatic domains including telomeres are also peripherally localized, but there is no evidence that yeast have lamins or a fibrous nuclear envelope scaffold. Nonetheless, we found that the Lem2 and Mani proteins of the fission yeast Schizosaccharomyces pombe, evolutionarily distant relatives of the Lap2/Emerin/ Mani (LEM) sub-family of animal cell lamin-associated proteins, perform fundamental functions of the animal cell lamina. These integral inner nuclear membrane localized proteins, with nuclear localized DNA binding Helix-Extension-Helix (HEH) domains, impact nuclear envelope structure and integrity, are essential for the enrichment of telomeres at the nuclear periphery and by means of their HEH domains anchor chromatin, most likely transcriptionally repressed heterochromatin, to the nuclear periphery. These data indicate that the core functions of the nuclear lamina are conserved between fungi and animal cells and can be performed in fission yeast, without lamins or other intermediate filament proteins.
机译：在动物细胞中，核纤层素由核纤层蛋白和核纤层蛋白相关蛋白组成，具有多种功能：它为核被膜提供了结构支架，并为核外围提供了束缚蛋白和异染色质。在酵母中，蛋白质和包括端粒的大杂色结构域也位于外围，但是没有证据表明酵母具有lamins或纤维状核被膜支架。尽管如此，我们发现裂变酵母粟酒裂殖酵母的Lem2和Mani蛋白是与动物细胞层粘连蛋白相关的Lap2 / Emerin / Mani（LEM）子家族的进化远亲，具有动物细胞层板的基本功能。这些完整的内在核膜定位蛋白，具有结合核螺旋DNA的螺旋-延伸-螺旋（HEH）结构域，影响核被膜结构和完整性，对于富集核外围的端粒及其通过HEH结构域锚定染色质至关重要，最有可能转录抑制异染色质，到核外围。这些数据表明核层板的核心功能在真菌和动物细胞之间是保守的，并且可以在裂变酵母中执行，而没有层板蛋白或其他中间丝蛋白。

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