...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >ANALYSIS OF RATE-DETERMINING CONFORMATIONAL CHANGES DURING SELF-SPLICING OF THE TETRAHYMENA INTRON
【24h】

ANALYSIS OF RATE-DETERMINING CONFORMATIONAL CHANGES DURING SELF-SPLICING OF THE TETRAHYMENA INTRON

机译：四面膜内膜自粘过程中确定速率的构象变化分析

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

RNA catalyzed reactions are often limited in vitro by the rate of structural rearrangements in the RNA. Analysis of intra- and intermolecular splicing of the Tetrahymena preribosomal RNA revealed two well resolved kinetic phases with rate constants of approximately 2.5 and 0.02 min(-1) at 30 degrees C. The data are consistent with a model in which the second phase results from slow refolding of the pre-rRNA. Point mutations result in redistribution of the RNA among different conformations that can be detected by native gel electrophoresis. The active pre-rRNA rapidly progresses to a product complex in the presence of GTP. Release of the ligated exons is slightly slower than splicing at 30 degrees C (0.3 -0.5 min(-1)). In contrast, the intermediate complex after the first step of splicing dissociates much more slowly (5 x 10(-3) min(-1)), accounting for the low amount of intron-3' exon intermediate typically seen during splicing of wild type pre-rRNA. These results provide an initial framework for studying conformational changes that accompany excision of the Tetrahymena intron from ribosomal RNA.
机译：RNA催化的反应通常在体外受到RNA中结构重排速率的限制。四膜虫前核糖体RNA的分子内和分子间剪接的分析显示，在30摄氏度时，两个分辨良好的动力学相的速率常数分别约为2.5和0.02 min（-1）。数据与其中第二相来自以下模型的模型一致前rRNA的缓慢重折叠。点突变导致RNA在不同构象之间重新分布，可以通过天然凝胶电泳检测到。在存在GTP的情况下，活性pre-rRNA迅速发展为产物复合物。连接的外显子的释放比在30摄氏度（0.3 -0.5分钟（-1））进行剪接要慢一些。相比之下，第一步剪接后的中间体复合物解离的速度要慢得多（5 x 10（-3）min（-1）），这说明野生型剪接过程中通常见到的内含子3'外显子中间体含量低前rRNA。这些结果提供了一个初步的框架，用于研究伴随核糖体RNA切除四膜虫内含子的构象变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号