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首页> 外文期刊>Cell motility and the cytoskeleton >Analysis of the linkage of MYRIP and MYO7A to melanosomes by RAB27A in retinal pigment epithelial cells.
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Analysis of the linkage of MYRIP and MYO7A to melanosomes by RAB27A in retinal pigment epithelial cells.

机译：视网膜色素上皮细胞中RAB27A分析MYRIP和MYO7A与黑素体的连接。

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摘要

The apical region of the retinal pigment epithelium (RPE) typically contains melanosomes. Their apical distribution is dependent on RAB27A and the unconventional myosin, MYO7A. Evidence from studies using in vitro binding assays, melanocyte transfection, and immunolocalization have indicated that the exophilin, MYRIP, links RAB27A on melanosomes to MYO7A, analogous to the manner that melanophilin links RAB27A on melanocyte melanosomes to MYO5A. To test the functionality of this hypothesis in RPE cells, we have examined the relationship among MYRIP, RAB27A and MYO7A with studies of RPE cells in primary culture (including live-cell imaging), analyses of mutant mouse retinas, and RPE cell fractionation experiments. Our results indicate that the retinal distribution of MYRIP is limited to the RPE, mainly the apical region. In RPE cells, RAB27A, MYRIP, and MYO7A were all associated with melanosomes, undergoing both slow and rapid movements. Analyses of mutant mice provide genetic evidence that MYRIP is linked to melanosomes via RAB27A, but show that recruitment of MYRIP to apical RPE is independent of melanosomes and RAB27A. RAB27A and MYRIP also associated with motile small vesicles of unknown origin. The present results provide evidence from live RPE cells that the RAB27A-MYRIP-MYO7A complex functions in melanosome motility. They also demonstrate that RAB27A provides an essential link to the melanosome.
机译：视网膜色素上皮（RPE）的顶端区域通常包含黑素体。它们的根尖分布取决于RAB27A和非常规的肌球蛋白MYO7A。使用体外结合测定，黑素细胞转染和免疫定位的研究证据表明，外泌素MYRIP将黑素体上的RAB27A与MYO7A连接，类似于黑素蛋白将黑素体上的RAB27A与黑素细胞黑素与MYO5A连接的方式。为了测试RPE细胞中这一假设的功能，我们检查了MYRIP，RAB27A和MYO7A与原代培养中RPE细胞的研究（包括活细胞成像），突变小鼠视网膜的分析以及RPE细胞分级实验之间的关系。我们的结果表明，MYRIP的视网膜分布仅限于RPE，主要是顶端区域。在RPE细胞中，RAB27A，MYRIP和MYO7A均与黑素体相关，并经历缓慢和快速运动。突变小鼠的分析提供了遗传证据，证明MYRIP通过RAB27A与黑素体相连，但表明MYRIP募集到顶端RPE并不依赖于黑素体和RAB27A。 RAB27A和MYRIP也与来源不明的活动性小囊泡有关。本结果提供了来自活RPE细胞的证据，表明RAB27A-MYRIP-MYO7A复合物在黑素体运动中起作用。他们还证明了RAB27A提供了与黑素体的必要连接。

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