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首页> 外文期刊>Cell motility and the cytoskeleton >Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells.
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Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells.

机译：应力纤维远离粘着斑的移动将粘着斑识别为固定细胞中应力纤维集合的部位。

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Force generated in contractile actin filament bundles (stress fibers-SFs) is transmitted to the extracellular matrix (ECM) via linker proteins and transmembrane integrins at focal adhesions (FAs). Though it has long been known that actin is rapidly exchanged in FAs, the connection between SFs and FAs has not been studied in detail. We introduced fiduciary marks on SFs by expressing GFP-palladin or GFP-alpha-actinin-1, which are both FA and dense body proteins, and by pattern bleaching of GFP-actin. Following fiduciary marks on SFs over time by time-lapse fluorescence microscopy, we detected assembly of SFs at FAs in stationary cells resulting in movement of SFs away from FAs with a velocity of 0.2-0.4 microm/min. Visualization of FAs in GFP-palladin/DsRed-paxillin double transfected cells showed that SF elongation was not accompanied by a change in FA length. SF elongation at FAs depended on actin polymerization and force as demonstrated by inhibitors of actin polymerization (cytochalasin D, jasplakinolide) and inhibitors of myosin-dependent contraction (blebbistatin, Y-27632), respectively. Our finding of SF assembly at FAs has important implications for SF formation, force transmission, and tension distribution within the actin cytoskeletal network of stationary cells.
机译：收缩性肌动蛋白丝束（应力纤维-SFs）中产生的力通过接头蛋白和跨膜整联蛋白以粘着斑（FAs）的形式传递到细胞外基质（ECM）。尽管早就知道肌动蛋白在FA中快速交换，但尚未详细研究SF与FA之间的连接。我们通过表达既是FA又是致密体蛋白的GFP-palladin或GFP-alpha-actinin-1，并通过GFP-actin的模式漂白，在SF上引入了基准标记。通过随时间推移的荧光显微镜对SFs进行基准标记，我们检测到SFs在固定细胞中FAs处的组装，导致SFs以0.2-0.4 microm / min的速度从FAs移开。 GFP-palladin / DsRed-paxillin双重转染细胞中FAs的可视化表明SF延长不伴随FA长度的改变。分别由肌动蛋白聚合抑制剂（细胞松弛素D，茉莉花素）和肌球蛋白依赖性收缩抑制剂（blebbistatin，Y-27632）证明，FAs上的SF伸长取决于肌动蛋白聚合和作用力。我们在FA上发现SF组装对于SF的形成，力传递以及固定细胞的肌动蛋白细胞骨架网络内的张力分布具有重要意义。

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