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首页> 外文期刊>Cell motility and the cytoskeleton >Connexin 43 is localized with actin in tenocytes.
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Connexin 43 is localized with actin in tenocytes.

机译：连接蛋白43被肌动蛋白定位于肌腱细胞中。

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摘要

Varieties of cell-matrix or cell-cell adhesions are associated with the actin cytoskeleton. However, for gap junctions, which are both channels and adhesions, there has been little evidence for such an association. The purpose of this study was to determine if connexin 43 (Cx43) associates with actin and to determine if this association is altered under mechanical load in tenocytes, a mechanically sensitive cell. Avian tenocytes were subjected to multiple cyclic strain regimens and then fixed and examined immunohistochemically at various times poststrain to determine where Cx43 protein was localized within the cells in relation to actin filaments. Four percent of tenocytes had colocalization of actin filaments and Cx43, which was significantly increased with 5% cyclic strain. To confirm this phenomenon, human tenocytes and COS-7 cells were also subjected to cyclic strain and then fixed at the same times after strain. As with avian tenocytes, Cx43 was colocalized with actin in human tenocytes and COS-7 cells. The colocalization increased significantly after cyclic strain in human tenocytes but not in COS-7 cells. The lack of detectable changes in COS-7 cells may indicate that they are less mechanosensitive than tenocytes perhaps due to the less robust actin cytoskeleton seen in the COS-7 cells when compared to the tenocytes. Furthermore, inhibiting myosin II activity greatly reduced the immunohistochemically-detectable Cx43 on actin filaments. Connexins may associate with actin to stabilize gap junctions at the plasma membrane, ensuring that tenocytes remain coupled during periods of prolonged or intense mechanical loading.
机译：细胞基质或细胞间粘附的各种变化与肌动蛋白的细胞骨架有关。但是，对于间隙连接（既是通道又是粘附），几乎没有证据表明这种关联。这项研究的目的是确定连接蛋白43（Cx43）是否与肌动蛋白结合，并确定这种结合在肌腱细胞（一种机械敏感性细胞）的机械负荷下是否发生改变。禽肌腱细胞经历了多种循环应变方案，然后在训练后的不同时间进行固定和免疫组织化学检查，以确定相对于肌动蛋白丝，Cx43蛋白在细胞内的位置。百分之四的肌腱细胞具有肌动蛋白丝和Cx43的共定位，并在5％的循环应变下显着增加。为了证实这种现象，人类肌腱细胞和COS-7细胞也经历了循环应变，然后在应变后的同一时间固定。与禽肌腱细胞一样，Cx43与肌动蛋白在人肌腱细胞和COS-7细胞中共定位。循环应变后，人肌腱细胞中的共定位显着增加，而COS-7细胞中则没有。 COS-7细胞中缺乏可检测到的变化可能表明它们比肌腱细胞对机械的敏感性更低，这可能是由于与肌腱细胞相比，COS-7细胞中肌动蛋白细胞骨架的强度较弱。此外，抑制肌球蛋白II的活性大大降低了肌动蛋白丝的免疫组织化学检测Cx43。连接蛋白可与肌动蛋白结合以稳定质膜上的间隙连接，从而确保肌腱细胞在长时间或强烈的机械负荷期间保持偶联状态。

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