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首页> 外文期刊>Cell motility and the cytoskeleton >The effect of combined simulated microgravity and microgrooved surface topography on fibroblasts.
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The effect of combined simulated microgravity and microgrooved surface topography on fibroblasts.

机译：模拟微重力和微槽表面形貌联合对成纤维细胞的影响。

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This study evaluated in vitro the differences in morphological behaviour between fibroblast cultured on smooth and microgrooved substrata (groove depth: 0.5 microm, width: 1, 2, 5, and 10 microm), which were subjected to simulated microgravity. The aim of the study was to clarify which of these parameters was more dominant to determine cell behaviour. Morphological characteristics were investigated using scanning electron microscopy and fluorescence microscopy in order to obtain qualitative information on cell alignment and area. Confocal laser scanning microscopy visualised distribution of actin filaments and focal adhesion points. Finally, expression of collagen type I, fibronectin, and alpha1- and beta1-integrin were investigated by PCR. Microscopy and image analysis showed that the fibroblasts aligned along the groove direction on all textured surfaces. On the smooth substrata, cells had spread out in a random fashion. The alignment of cells cultured on grooved surfaces decreased under simulated microgravity, especially after 24 h of culturing. Cell surface area on grooved substrata were significantly smaller than on smooth substrata, but simulated microgravity on the grooved groups resulted in an enlargement of cell area. ANOVA was performed on all main parameters: topography, gravity force, and time. In this analysis, all parameters proved significant. In addition, gene levels were reduced by microgravity particularly those of beta1-integrin and fibronectin. From our data it is concluded that the fibroblasts primarily adjust their shape according to morphological environmental cues like substratum surface whilst a secondary, but significant, role is played by microgravity conditions.
机译：本研究评估了体外培养的成纤维细胞在光滑和微沟槽基质（沟槽深度：0.5微米，宽度：1、2、5和10微米）上的形态学差异，并对其进行了模拟微重力处理。该研究的目的是弄清楚这些参数中的哪个参数在确定细胞行为方面更占优势。为了获得关于细胞排列和面积的定性信息，使用扫描电子显微镜和荧光显微镜研究了形态特征。共聚焦激光扫描显微镜可视化肌动蛋白丝和粘着点的分布。最后，通过PCR研究了I型胶原，纤连蛋白以及α1-和β1-整联蛋白的表达。显微镜和图像分析表明，成纤维细胞在所有纹理表面上沿凹槽方向排列。在光滑的基质上，细胞以随机的方式扩散。在模拟微重力下，尤其是在培养24小时后，在凹槽表面上培养的细胞的排列减少。开槽基质上的细胞表面积明显小于光滑基质上的细胞表面积，但开槽基团上的模拟微重力导致了细胞面积的扩大。对所有主要参数进行了方差分析：地形，重力和时间。在此分析中，所有参数均证明是有效的。另外，基因水平由于微重力而降低，特别是β1-整合素和纤连蛋白的水平。从我们的数据得出的结论是，成纤维细胞主要根据形态环境提示（如基质表面）调整形状，而微重力条件则起着次要但重要的作用。

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