...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell motility and the cytoskeleton >Localization of wild type and mutant class I myosin proteins in Aspergillus nidulans using GFP-fusion proteins.
【24h】

Localization of wild type and mutant class I myosin proteins in Aspergillus nidulans using GFP-fusion proteins.

机译：使用GFP融合蛋白在野生曲霉中定位野生型和突变I类肌球蛋白。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have examined the distribution of MYOA, the class I myosin protein of the filamentous fungus Aspergillus nidulans, as a GFP fusion protein. Wild type GFP-MYOA expressed from the myoA promoter is able to rescue a conditional myoA null mutant. Growth of a strain expressing GFP-MYOA as the only class I myosin was approximately 50% that of a control strain, demonstrating that the fusion protein retains substantial myosin function. The distribution of the wild type GFP-MYOA fusion is enriched in growing hyphal tips and at sites of septum formation. In addition, we find that GFP-MYOA is also found in patches at the cell cortex. We have also investigated the effects of deletion or truncation mutations in the tail domain on MYOA localization. Mutant GFP-MYOA fusions that lacked either the C-terminal SH3 or a portion of the C-terminal proline-rich domain had subcellular distributions like wild type MYOA, consistent with their ability to complement a myoA null mutant. In contrast, mutants lacking all of the C-terminal proline-rich domain or the TH-1-like domain were mainly localized diffusely throughout the cytoplasm, but could less frequently be found in patches, and were unable to complement a myoA null mutant. The GFP-MYOA DeltaIQ mutant was localized into large bright fluorescent patches in the cytoplasm. This mutant protein was subsequently found to be insoluble. Copyright 2000 Wiley-Liss, Inc.
机译：我们已经检查了MYOA（丝状真菌构巢曲霉的I类肌球蛋白）作为GFP融合蛋白的分布。从myoA启动子表达的野生型GFP-MYOA能够挽救条件性myoA无效突变体。表达GFP-MYOA作为唯一的I类肌球蛋白的菌株的生长约为对照菌株的50％，这表明融合蛋白保留了基本的肌球蛋白功能。野生型GFP-MYOA融合体的分布富集在生长的菌丝尖端和隔膜形成部位。另外，我们发现GFP-MYOA也在细胞皮层的斑块中被发现。我们还研究了尾部结构域的缺失或截短突变对MYOA定位的影响。缺少C末端SH3或C末端富含脯氨酸的结构域的一部分的突变GFP-MYOA融合体具有像野生型MYOA一样的亚细胞分布，与它们补充myoA无效突变体的能力一致。相比之下，缺乏所有C末端富含脯氨酸的结构域或TH-1样结构域的突变体主要分散在整个细胞质中，但在补丁中的出现频率较低，并且无法与myoA空突变体互补。 GFP-MYOA DeltaIQ突变体位于细胞质中大的明亮荧光斑块中。随后发现该突变蛋白是不溶的。版权所有2000 Wiley-Liss，Inc.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号