Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA

Yanwei Jia; Pui-ln Mak; Conner Massey

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Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA

机译：使用干燥的试剂构建微流控芯片，用于LATE-PCR扩增和单链DNA检测

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LATE-PCR is an advanced form of non-symmetric PCR that efficiently generates single-stranded DNA which can readily be characterized at the end of amplification by hybridization to low-temperature fluorescent probes. We demonstrate here for the first time that monoplex and duplex LATE-PCR amplification and probe target hybridization can be carried out in double layered PDMS microfluidics chips containing dried reagents. Addition of a set of reagents during dry down overcomes the common problem of single-stranded oligonucleotide binding to PDMS. These proof-of-principle results open the way to construction of inexpensive point-of-care devices that take full advantage of the analytical power of assays built using LATE-PCR and low-temperature probes.

机译：LATE-PCR是非对称PCR的一种高级形式，可有效生成单链DNA，在扩增结束时可通过与低温荧光探针杂交轻松表征其特征。我们在此首次证明，可以在包含干燥试剂的双层PDMS微流控芯片中进行单重和双工LATE-PCR扩增以及探针靶标杂交。在干燥过程中添加一组试剂克服了单链寡核苷酸与PDMS结合的普遍问题。这些原理证明的结果为构建廉价的即时护理设备开辟了道路，该设备充分利用了使用LATE-PCR和低温探针构建的测定的分析能力。

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《Lab on a chip》 |2013年第23期|共7页
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Yanwei Jia; Pui-ln Mak; Conner Massey;
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正文语种 eng
中图分类化学实验（实验化学）;生物化学;生物科学;化学;
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1. Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA [J] . Yanwei Jia, Pui-ln Mak, Conner Massey Lab on a chip . 2013,第23期

机译：使用干燥的试剂构建微流控芯片，用于LATE-PCR扩增和单链DNA检测
2. Detection of influenza virus using a lateral flow immunoassay for amplified DNA by a microfluidic RT-PCR chip [J] . Nagatani N., Yamanaka K., Ushijima H., The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry . 2012,第15期

机译：使用微流控RT-PCR芯片的侧向流免疫法对扩增的DNA进行流感病毒检测
3. An integrated microfluidic chip for DNA/RNA amplification, electrophoresis separation and on-line optical detection [J] . Huang FC, Liao CS, Lee GB Electrophoresis: The Official Journal of the International Electrophoresis Society . 2006,第16期

机译：集成的微流体芯片，用于DNA / RNA扩增，电泳分离和在线光学检测
4. Microfluidic chips for DNA amplification, electrophoresis separation and on-line optical detection [C] . Gwo-Bin Lee, Che-Hsin Lin, Fu-Chun Huang, IEEE Annual International Conference on Micro Electro Mechanical Systems . 2003

机译：用于DNA扩增的微流体芯片，电泳分离和在线光学检测
5. DNA Amplification and Detection in Micro/nano-fluidic Chip. [D] . Wu, Jinbo. 2011

机译：微/纳米流体芯片中的DNA扩增和检测。
6. An LED-Driven AuNPs-PDMS Microfluidic Chip and Integrated Device for the Detection of Digital Loop-Mediated Isothermal DNA Amplification [O] . Zengming Zhang, Shuhao Zhao, Fei Hu, 2020

机译：LED驱动的AuNPs-PDMS微流控芯片和集成设备用于检测数字环介导的等温DNA扩增
7. P1DH.4 - Miniaturized System with a Facile Microfluidic Chip for DNA Extraction, Isothermal Amplification and Fluorescence Detection [O] . X. Chen, L. Zhang, D. Cui 2018

机译：P1DH.4 - 具有舒适微流体芯片的小型化系统，用于DNA提取，等温扩增和荧光检测

Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA

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