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AUTOMATED PROCEDURE FOR ESTIMATION OF BLOOD CHOLINESTERASE ACTIVITIES IN RABBITS

机译:估计兔胆汁中胆固醇胆固醇活性的自动程序

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An automated procedure for estimation of blood cholinesterase activities was evaluated and used to determine baseline cholinesterase activities in rabbits, In addition, the kinetics of cholinesterase inhibition and recovery from diazinon challenge were determined by use of this procedure, The procedure developed is a modification of the colorimetric Ellman technique, adapted for use with the Instrumentation Laboratory Multistat III Plus Centrifugal Analyzer, The substrate used in this technique was acetylthiocholine, Baseline cholinesterase activities in domestic rabbits were plasma cholinesterase, 360 mU/ml; red blood cell cholinesterase, 2,658 mU/ml; and whole blood cholinesterase, 1,332 mU/ml, Rabbits were challenged with 0.3 LD50 (40 mg/kg) of diazinon, and blood samples were obtained at hourly, then daily intervals, Cholinesterase activity in these samples was determined to monitor the effect of the cholinesterase inhibitor diazinon in vivo, as estimated by the Multistat III Plus Centrifugal Analyzer procedure, Plasma cholinesterase activity decreased to 4% within 6 h, whereas red blood cell cholinesterase activity decreased to 51%, By 10 days, plasma and red blood cell cholinesterase activities had returned to 100 and 91%, respectively, of pre-challenge activities.
机译:评估了自动评估血液胆碱酯酶活性的方法,并用于确定兔子的基线胆碱酯酶活性。此外,通过使用该方法确定了胆碱酯酶抑制动力学和从二嗪农攻击中恢复。比色Ellman技术,适用于仪器实验室Multistat III Plus离心分析仪,该技术使用的底物为乙酰硫代胆碱,家兔的基线胆碱酯酶活性为血浆胆碱酯酶,360 mU / ml。红细胞胆碱酯酶,2,658 mU / ml;和全血胆碱酯酶1,332 mU / ml,用0.3 LD50(40 mg / kg)的二嗪农攻击兔子,每小时采集一次血样,然后每天间隔,测定这些样品中的胆碱酯酶活性以监测血脂的影响。根据Multistat III Plus离心分析仪程序估算,体内胆碱酯酶抑制剂二嗪农在6小时内血浆胆碱酯酶活性降至4%,而红细胞胆碱酯酶活性降至51%,到10天时血浆和红细胞胆碱酯酶活性已分别恢复到挑战前活动的100%和91%。

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