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首页> 外文期刊>Laboratory Animal Science >IN VITRO FERTILIZATION OF FOLLICULAR OOCYTES BY FROZEN-THAWED SPERMATOZOA IN JAPANESE MONKEYS (MACACA FUSCATA)
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IN VITRO FERTILIZATION OF FOLLICULAR OOCYTES BY FROZEN-THAWED SPERMATOZOA IN JAPANESE MONKEYS (MACACA FUSCATA)

机译：冻融过的精子在日本猕猴中卵泡卵的体外受精

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摘要

We report the cryopreservation of epididymal spermatozoa and in vitro fertilization for Japanese monkeys (Macaca fuscata). Ovarian stimulation of seven monkeys was studied by the administration of equine chorionic gonadotrophin (eCG) and human chorionic gonadotrophin (hCG) during the nonbreeding season, after which oocytes were collected from ovarian follicles. The oocytes were subjected to in vitro fertilization by the frozen-thawed epididymal spermatozoa collected from a male monkey during the nonbreeding season. Before the experiment the epididymides and oocytes were transported about 500 km, during which the epididymides were kept in mineral oil to prevent drying and the oocytes were kept in a maturation medium. This technique of cryopreservation and in vitro fertilization has a wide applicability in nonhuman primate reproduction, including the treatment of infertility and the preservation of wild, nonhuman primates.
机译：我们报告冷冻保存的附睾精子和日本猴（猕猴）的体外受精。通过在非繁殖季节施用马绒毛膜促性腺激素（eCG）和人绒毛膜促性腺激素（hCG）研究了七只猴子的卵巢刺激，然后从卵巢卵泡中收集卵母细胞。在非繁殖季节，通过从雄猴收集的冻融的附睾精子对卵母细胞进行体外受精。在实验之前，将附睾和卵母细胞运输约500 km，在此过程中，将附睾被保存在矿物油中以防止干燥，并将卵母细胞保存在成熟培养基中。冷冻保存和体外受精的这项技术在非人类灵长类动物的繁殖中具有广泛的适用性，包括不育症的治疗和野生的非人类灵长类动物的保存。

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