...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Zygote >Impact of in vitro fertilization of bovine oocytes with sex-sorted frozen-thawed spermatozoa on developmental kinetics, quality and sex ratio of developing embryos
【24h】

Impact of in vitro fertilization of bovine oocytes with sex-sorted frozen-thawed spermatozoa on developmental kinetics, quality and sex ratio of developing embryos

机译：性别解冻的精子对牛卵母细胞的体外受精对发育中胚胎发育动力学，质量和性别比的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We studied whether bovine embryos developing after in vitro fertilization (IVF) with sex-sorted spermatozoa differed in developmental kinetics, quality and sex ratio from embryos produced with unsorted spermatozoa. Abattoir-derived oocytes were fertilized with X-sorted, Y-sorted or unsorted spermatozoa from a single bull. To evaluate economical use of the sex-sorted spermatozoa, washed spermatozoa from a single straw (2 million spermatozoa) were used to fertilize each batch of collected oocytes without any further isolation steps. Concentration of the unsorted spermatozoa was adjusted accordingly. Fertilizations were assessed by staining sperm asters at 10 hpi and pronuclei at 20 hpi. Embryo development and morphological quality were monitored on days 2, 7, 8 and 9 of the development (IVF = day 0). All embryos were sexed using PCR. Following fertilization, penetration and subsequent cleavage rates were compromised in the X-sorted group compared with the Y-sorted and unsorted groups (penetration: 58.0% vs. 89.8% and 90.0%, cleavage: 65.3% vs. 81.5% and 75.0%). The use of the sex-sorted spermatozoa did not, however, reduce the proportion of transferable embryos (sex-sorted 29.6% vs. unsorted 27.7%) or their quality (quality 1: sex-sorted 36.0% vs. unsorted 19.9%). The Y-sorted spermatozoa produced more transferable embryos of better quality than the X-sorted spermatozoa (days 7-8: 31.9% vs. 26.4%, quality 1: 38.9% vs. 30.6%). On average, out of 10 transferable embryos, nine were of the predicted sex in the X- and Y-sorted spermatozoa groups. These results indicate that low numbers of X- and Y-sorted spermatozoa can be used successfully for female and male embryo production in vitro.
机译：我们研究了在体外受精（IVF）后，按性别分选的精子发育的牛胚胎在发育动力学，质量和性别比方面与未分选的精子产生的胚胎是否存在差异。屠宰场衍生的卵母细胞用来自一头公牛的X分选，Y分选或未分选的精子受精。为了评估按性别分选的精子的经济用途，可使用单根秸秆（200万个精子）中洗过的精子对每批收集的卵母细胞进行受精，而无需任何进一步的分离步骤。相应地调整未分选精子的浓度。通过在10 hpi染色精子和在20 hpi染色核来评估受精情况。在发育的第2、7、8和9天（IVF =第0天）监测胚胎的发育和形态质量。使用PCR对所有胚胎进行性别鉴定。施肥后，与Y分选组和未分选组相比，X分选组的穿透率和随后的分裂率均受到损害（穿透率：58.0％vs. 89.8％和90.0％，分裂：65.3％vs. 81.5％和75.0％） 。但是，按性别分类的精子的使用并没有减少可转移胚胎的比例（性别分类的29.6％对比未分类的27.7％）或它们的质量（质量1：按性别分类的36.0％对比未分类的19.9％）。与X排序的精子相比，Y排序的精子可产生更多质量更好的可移植胚胎（7-8天：31.9％对26.4％，质量1：38.9％对30.6％）。平均而言，在10个可移植胚胎中，有9个在X和Y分类的精子群中具有预期的性别。这些结果表明，少量的X和Y分选的精子可成功用于体外雌，雄胚胎的生产。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号