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Method for continuous infusion into the portal vein of mice

机译：连续输注小鼠门静脉的方法

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Recombinant retroviral vectors are attractive for in vivo gene transfer into the liver because they integrate into the host-cell genome, resulting in permanent gene expression. Gene-transfer efficiency can be improved by increasing the number of retroviral particles delivered to hepatocytes. For this purpose, we report a mouse model for continuous infusion into the portal circulation permitting large-volume vector administration, which will allow marked increase in gene-transfer efficiency. Continuous saline infusion was evaluated, using various parameters, and an infusion rate of 6 ml/24 h was found safe and well tolerated for at least 2 weeks. No significant changes in liver and kidney function and electrolyte balance were observed during the infusion. In addition to providing a valuable method for in vivo hepatic gene therapy, this model has a number of other potential applications, including mouse studies of hepatic tumor therapy, pharmacology, toxicology, and liver biology.
机译：重组逆转录病毒载体对于将体内基因转移到肝脏具有吸引力，因为它们整合到宿主细胞基因组中，从而导致永久性基因表达。通过增加传递到肝细胞的逆转录病毒颗粒的数量可以提高基因转移效率。为此，我们报告了一种小鼠模型，可将其连续输注至门脉循环中，从而可进行大量载体给药，这将大大提高基因转移效率。使用各种参数对连续输注盐水进行了评估，发现输注速度为6 ml / 24 h是安全的，并且可以耐受至少2周。输注过程中未观察到肝肾功能和电解质平衡的显着变化。除了为体内肝基因治疗提供有价值的方法外，该模型还具有许多其他潜在应用，包括对肝肿瘤治疗，药理学，毒理学和肝生物学的小鼠研究。

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