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首页> 外文期刊>Cell motility and the cytoskeleton >Adhesion, alignment, and migration of cultured Schwann cells on ultrathin fibronectin fibres.
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Adhesion, alignment, and migration of cultured Schwann cells on ultrathin fibronectin fibres.

机译：培养的雪旺氏细胞在超薄纤连蛋白纤维上的粘附，排列和迁移。

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摘要

Individual fibres of fibronectin (Fn-fibres), an extracellular matrix cell adhesion glycoprotein, were produced from a purified solution of fibronectin. These fibres range from 0.5-7 microm in width and have been engineered to produce mats (Fn-mats) by using a unidirectional shear force to orientate the fibres. Fn-fibres have been shown to promote alignment by contact guidance of human dermal fibroblasts, neurites, macrophages, and epitenon fibroblasts. Fn-mats have been used to orientate and enhance the regeneration of peripheral nerve components. We investigated cell spreading, orientation, formation of focal contacts, and the speed of cell movement on individual Fn-fibres, glass-covered with poly-L-lysine and poly-L-lysine/laminin/Fn. Fibronectin fibres significantly promoted cell spreading and the speed of cell migration with alignment of focal contacts and F-actin filaments to the axis of the fibres. The study reveals the potential of Fn-fibres to guide and direct cellular behaviour by contact guidance. The increase in migration and other behaviour exhibited by Schwann cells on Fn-fibres justifies the use of Fn-mats for peripheral nerve repair and is clinically important in that atrophy of the target organ, which is the most common failure of nerve repair, may be minimised.
机译：纤连蛋白的单纤维（Fn-纤维）是一种细胞外基质细胞粘附糖蛋白，由纯化的纤连蛋白溶液制成。这些纤维的宽度范围为0.5-7微米，并且已设计为通过使用单向剪切力使纤维定向来生产毡垫（Fn-mats）。 Fn纤维已显示出通过人类皮肤成纤维细胞，神经突，巨噬细胞和表皮成纤维细胞的接触引导而促进排列。 Fn-mats已被用于定向和增强周围神经成分的再生。我们调查了玻璃纤维覆盖的聚L-赖氨酸和聚L-赖氨酸/层粘连蛋白/ Fn覆盖的单个Fn纤维上的细胞扩散，方向，焦点接触的形成以及细胞运动的速度。纤连蛋白纤维通过聚焦接触和F-肌动蛋白丝与纤维轴对齐，显着促进细胞扩散和细胞迁移速度。这项研究揭示了Fn纤维通过接触引导来指导和指导细胞行为的潜力。雪旺氏细胞在Fn纤维上表现出的迁移和其他行为的增加证明了Fn-mats用于周围神经修复的合理性，并且在临床上很重要，因为目标器官的萎缩可能是神经修复最常见的失败。最小化。

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