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NDP kinase moves into developing primary cilia.

机译：NDP激酶进入发育中的初级纤毛。

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摘要

Inmunofluorescence staining of murine NIH3T3 fibroblasts grown at high density shows that conventional nucleoside diphosphate (NDP) kinases A and B localize to a sensory organelle, the primary cilium. Similar results are obtained with Xenopus A6 kidney epithelial cells, suggesting that NDP kinases are a universal component of the primary cilium. The translocation of NDP kinase into primary cilia depends on size, taking place only when cilia reach a critical length of 5-6 microm. In mature cilia, NDP kinases are distributed along the ciliary shaft in a punctate pattern that is distinct from the continuous staining observed with acetylated alpha-tubulin, a ciliary marker and axonemal component. Isolation of a fraction enriched in primary cilia from A6 cells led to the finding that ciliary NDP kinase is enzymatically active, and is associated with the membrane and the matrix, but not the axoneme. In contrast, acetylated alpha-tubulin is found in the axoneme and, to a lesser extent, in the membrane. Based on the tightly regulated translocation process and the subciliary distribution pattern of NDP kinase, we propose that it plays a role in the elongation and maintenance of primary cilia by its ability to regenerate the GTP utilized by ciliary microtubule turnover and transmembrane signaling. Cell Motil. Cytoskeleton 59:62-73, 2004. Copyright 2004 Wiley-Liss, Inc.
机译：高密度生长的鼠NIH3T3成纤维细胞的免疫荧光染色表明，常规的核苷二磷酸（NDP）激酶A和B定位于感觉细胞器，即初级纤毛。非洲爪蟾A6肾上皮细胞获得了相似的结果，表明NDP激酶是初级纤毛的通用成分。 NDP激酶向原发纤毛的转运取决于大小，只有在纤毛达到5-6微米的临界长度时才会发生。在成熟的纤毛中，NDP激酶以点状分布在睫状轴上，这与乙酰化的α-微管蛋白，睫状体标记物和轴突成分的连续染色不同。从A6细胞中分离出富含初级纤毛的级分，导致发现纤毛NDP激酶具有酶活性，并且与膜和基质有关，但与轴突无关。相反，在轴突中发现乙酰化的α-微管蛋白，而在膜中则较小。基于严格调控的易位过程和NDP激酶的亚睫状分布模式，我们认为它在纤毛的延长和维持中起着重要作用，因为它具有再生睫状微管更新和跨膜信号转导的GTP的能力。细胞动力。 Cytoskeleton 59：62-73，2004。2004年Wiley-Liss，Inc.版权所有。

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