DEMONSTRATION OF A BETA-TYPE ADAPTIN AT THE PLANT PLASMA MEMBRANE

Drucker M; Herkt B; Robinson DG

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DEMONSTRATION OF A BETA-TYPE ADAPTIN AT THE PLANT PLASMA MEMBRANE

机译：植物血浆膜上β-型阿帕丁的论证

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Highly purified plasma membrane was prepared from etiolated zucchini hypocotyls, developing pea cotyledons and suspension-cultured soybean cells by phase partitioning and sucrose density gradient centrifugation. The brain beta-adaptin monoclonal antibody B1/M6, which recognizes a similar adaptin in zucchini clathrin coated vesicles, cross-reacted with an electrophoretically identical polypeptide in the plant plasma membrane fractions. Immunogold labelling of thin sections of soybean cells confirmed the presence of the beta-adaptin at the plasma membrane. The presence of this adaptin at the Golgi apparatus could not be demonstrated unequivocally.

机译：通过相分离和蔗糖密度梯度离心，从黄化的西葫芦下胚轴，发育中的豌豆子叶和悬浮培养的大豆细胞中制备高纯度的质膜。脑β-adaptin单克隆抗体B1 / M6，在西葫芦网格蛋白包被的囊泡中识别相似的adaptin，与植物质膜级分中的电泳相同多肽发生交叉反应。大豆细胞薄片的免疫金标记证实了质膜上存在β-adaptin。高尔基体中这种衔接蛋白的存在不能被明确地证明。

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《Cell biology international.》 |1995年第3期|共11页
作者
Drucker M; Herkt B; Robinson DG;
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正文语种 eng
中图分类细胞生物学;
关键词
Brain coated vesicles; Clathrin; Complexes; Proteins; Polypeptides; Protoplasts; Golgi; Cells;

机译：脑包膜囊泡;网格蛋白;复合物;蛋白质;多肽;原生质体;高尔基体;细胞;

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专利

1. DEMONSTRATION OF A BETA-TYPE ADAPTIN AT THE PLANT PLASMA MEMBRANE [J] . Drucker M, Herkt B, Robinson DG Cell biology international. . 1995,第3期

机译：植物血浆膜上β-型阿帕丁的论证
2. Membrane distillation: Solar and waste heat driven demonstration plants for desalination [J] . R. Schwantes, A. Cipollina, F. Gross Desalination: The International Journal on the Science and Technology of Desalting and Water Purification . 2013,第Null期

机译：膜蒸馏：太阳能和废热驱动的脱盐示范工厂
3. PEM water electrolysers: evidence for membrane failure in 100 kW demonstration plants [J] . S. Stucki, G. G. Scherer, S. Schlagowski, Journal of Applied Electrochemistry . 1998,第10期

机译：PEM水电解槽：100 kW示范工厂中膜故障的证据
4. Extending the application of plasma electrolytic oxide coatings to novel low-rigidity beta-type titanium alloys: applications to load-bearing orthopaedic implants [C] . Mehdi Golozar, Constantin-Edi Tanase, Roger Brooks, World biomaterials congress . 2016

机译：将等离子体电解氧化物涂层的应用扩展到新型低刚度β型钛合金：在承重骨科植入物中的应用
5. The plasma membrane proton ATPase is an important determinant of salt tolerance in plants. [D] . Niu, Xiaomu. 1994

机译：质膜质子ATP酶是植物耐盐性的重要决定因素。
6. Adaptor self-aggregation adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits. [O] . M P Chang, W G Mallet, K E Mostov, 1993

机译：衔接子的自我聚集衔接子-受体的识别以及α-adaptin亚基与质膜的结合有助于网格蛋白包被的凹坑的衔接子（AP2）组分的募集。
7. Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits. [O] . M.P. Chang, W.G. Mallet, K.E. Mostov, 1993

机译：适配器自聚集，适配器 - 受体识别和α-适应素亚基与血浆膜的结合有助于募集克拉林涂层凹坑的适配器（AP2）组分。

DEMONSTRATION OF A BETA-TYPE ADAPTIN AT THE PLANT PLASMA MEMBRANE

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