A CONFOCAL MICROSCOPIC EVALUATION OF THE EFFECTS OF INSULIN IMPRINTING ON THE BINDING OF CONCANAVALIN A BY TETRAHYMENA PYRIFORMIS

Kovacs P; Sundermann C; Estridge BH; Csaba G

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A CONFOCAL MICROSCOPIC EVALUATION OF THE EFFECTS OF INSULIN IMPRINTING ON THE BINDING OF CONCANAVALIN A BY TETRAHYMENA PYRIFORMIS

机译：共聚焦显微镜评价梨状四膜虫对胰岛素结合对伴刀豆球蛋白A结合作用的影响

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With confocal microscopy it is possible to study the Concanavalin A (Con A) binding characteristics of the surface and interior of a single cell by viewing optical sections. It was observed in Tetrahymena pyriformis that Con A bound both to the plasma membrane and to intracellular structures. Incubation of cells with a competing sugar a-methylmannopyranoside, decreased binding. Hormonal imprinting with insulin resulted in an increase in binding of Con A to the cell surface and a decrease in intracellular binding. It is possible that the intracellular binding sites may migrate to the plasma membrane.

机译：使用共聚焦显微镜，可以通过观察光学切片来研究单个细胞表面和内部的伴刀豆球蛋白A（Con A）结合特征。在梨形四膜虫中观察到Con A结合到质膜和细胞内结构。用竞争性糖α-甲基甘露吡喃糖苷孵育细胞会降低结合。胰岛素的激素印迹导致Con A与细胞表面结合的增加和细胞内结合的减少。细胞内结合位点可能迁移到质膜。

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《Cell biology international.》 |1995年第12期|共6页
作者
Kovacs P; Sundermann C; Estridge BH; Csaba G;
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正文语种 eng
中图分类细胞生物学;
关键词
Lectins; Cells;

机译：凝集素;细胞;

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1. A CONFOCAL MICROSCOPIC EVALUATION OF THE EFFECTS OF INSULIN IMPRINTING ON THE BINDING OF CONCANAVALIN A BY TETRAHYMENA PYRIFORMIS [J] . Kovacs P, Sundermann C, Estridge BH, Cell biology international. . 1995,第12期

机译：共聚焦显微镜评价梨状四膜虫对胰岛素结合对伴刀豆球蛋白A结合作用的影响
2. Insulin pretreatment (imprinting) produces elevated capacity in the insulin binding of Tetrahymena. Different binding by the cilia of the body and oral field [J] . A. K. Fül?p, G. Csaba Bioscience Reports . 1994,第6期

机译：胰岛素预处理（印记）可增强四膜虫的胰岛素结合能力。纤毛对身体和口腔的结合不同
3. Different Effects of Concanavalin A on Insulin Binding to Cultured Cells in Monolayers: "Blocking" and "Trapping" Effects of Concanavalin A [J] . Kenji Sorimachi Cell structure and function . 1983,第4期

机译：伴刀豆球蛋白A对胰岛素与单层培养细胞结合的不同作用：伴刀豆球蛋白A的“阻断”和“诱捕”作用
4. SIMS Analysis of TiO_2 Nanoparticle Effects on Tetrahymena pyriformis [C] . Tina B. Angerer, John S. Fletcher ASMS Conference on Mass Spectrometry and Allied Topics . 2014

机译：TiO_2纳米粒子对Tetrahymena Pyriformis作用的影响分析
5. Evaluation of serum insulin-like growth factor binding proteins and the insulin-like growth factor binding protein-2 locus for potential associations with growth, carcass merit and meat quality in beef cattle. [D] . Pagan, Melvin. 2002

机译：评价血清胰岛素样生长因子结合蛋白和胰岛素样生长因子结合蛋白2位点与肉牛生长，屠体价值和肉质的潜在关联。
6. Conjugation in Tetrahymena pyriformis. The effect of polylysine concanavalin A and bivalent metals on the conjugation process [O] . 1976

机译：在梨形四膜虫中的缀合。聚赖氨酸伴刀豆球蛋白A和二价金属对结合过程的影响
7. Different effects of concanavalin A on insulin binding to cultured cells in monolayers: "blocking" and "trapping" effects of concanavalin A. [O] . Kenji Sorimachi 1983

机译：康丹林A对单层培养细胞胰岛素结合的不同影响：“阻断”和“诱捕”对康丹林A的影响。
8. Differential Lethal Effects of Mixed Reactor Radiations on Logarithmic-Phase and Synchronized Tetrahymena Pyriformis [R] . Nachtwey, D. S. 1968

机译：混合反应器辐射对对数期和同步四膜虫的差异致死效应

A CONFOCAL MICROSCOPIC EVALUATION OF THE EFFECTS OF INSULIN IMPRINTING ON THE BINDING OF CONCANAVALIN A BY TETRAHYMENA PYRIFORMIS

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