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首页> 外文期刊>Cell biology international. >Molecular cloning, expression profile and functional implications of clusterin in the pituitary gland of helmeted guinea fowl (Numida meleagris).
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Molecular cloning, expression profile and functional implications of clusterin in the pituitary gland of helmeted guinea fowl (Numida meleagris).

机译：Clusterin在头盔式珍珠鸡（Numida meleagris）垂体中的分子克隆，表达谱和功能意义。

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摘要

Clusterin is shown to contain putative amphipathic alpha-helices that mediate hydrophobic interactions with numerous types of molecules and may be involved in clearance of cellular debris caused by cell injury or death. To assess this function in vivo, we have cloned the full-length cDNA encoding guinea fowl (Numida meleagris) clusterin and studied its synthesis and expression pattern in specific cell types in pituitary. Quantity of clusterin mRNA expressed in pituitary and endocrine tissues was quantified by real-time PCR. Highest levels were detected in gonads. In situ hybridization showed clusterin mRNA in endocrine cells and folliculostellate cells. Clusterin protein detected by immunohistochemistry was observed in endocrine cells, folliculostellate cells and in colloid. The expression pattern suggests that clusterin is produced by endocrine cells for cytoprotection. Degenerating endocrine cells are phagocytosed by folliculostellate cells and digested by their lysosomal enzymes. In folliculostellate cells clusterin interacts and aggregates with by-products of digestion that subsequently become stored in colloid.
机译：簇蛋白显示含有推测的两亲性α-螺旋，其介导与多种类型分子的疏水相互作用，并且可能参与清除由细胞损伤或死亡引起的细胞碎片。为了在体内评估该功能，我们克隆了编码珍珠鸡（Numida meleagris）clusterin的全长cDNA，并研究了其在垂体特定细胞类型中的合成和表达模式。垂体和内分泌组织中表达的簇蛋白mRNA的量通过实时PCR定量。在性腺中检测到最高水平。原位杂交显示内分泌细胞和卵泡星状细胞中簇蛋白mRNA。通过免疫组织化学检测到的Clusterin蛋白在内分泌细胞，卵泡细胞和胶体中观察到。该表达模式表明簇蛋白由内分泌细胞产生用于细胞保护。退化的内分泌细胞被卵泡细胞吞噬，并被其溶酶体酶消化。在卵泡状细胞中，聚集蛋白与消化副产物相互作用并聚集，随后消化副产物被储存在胶体中。

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